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Heterophile Agglutination

Item Value
Approval req'd? No
Available Stat? No
Test code MONO
Performed by Immunology
In House Availability Tuesday, Friday (day shift)
Method Latex particle agglutination
Container type Gold top
Amount to Collect 1 mL blood
Sample type Serum
Preferred volume 0.5 mL serum
Processing notes Freeze serum at -20C
Normal range Negative
Synonyms monospot
Turn around times 1-3 days
Additional information Heterophile antibodies are present in greater than 90% of cases of infectious mononucleosis at some point during acute illness. These antibodies are named for their ability to react with the so-called Paul-Bunnell antigen found on bovine red blood cells. This test uses highly purified antigen to provide high sensitivity and specificity for detection of heterophile antibodies due to acute EBV infection, thus differential absorption with guinea pig kidney (GPK) is not required. In cases of heterophile-negative infection with high clinical suspicion, EBV-specific antibodies (see entry for EB Virus Antibodies) may be performed as a follow-up test.
LOINC code 5213-4
Last Updated 10/2/2010 11:52:04 AM
Entry Number 498
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