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Herpes simplex, DNA
| Item | Value | ||||
|---|---|---|---|---|---|
| Approval req'd? | Infants under 1 month of age require approval of Pediatric ID service. | ||||
| Available Stat? | No | ||||
| Utilization Guidelines | CSF testing should not be ordered on an immuncompetent patient unless there is no other explanation for CSF findings AND there are > 5 cells/mm3 or a protein of > 50 mg/dL These criteria do not apply if the patient is immunocompromised. | ||||
| Test code | Amniotic fluid: P319 Other samples: P337 |
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| Test group | Herpes | ||||
| Performed by | Amniotic fluid: Focus Diagnostics Other samples: Viracor |
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| Sendout? | Yes | ||||
| Method | Real time PCR | ||||
| Container type |
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| Amount to Collect |
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| Sample type | CSF, amniotic fluid, EDTA plasma | ||||
| Preferred volume |
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| Min. Volume |
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| Processing notes | Test sent out and reported by Microbiology. CSF and amniotic fluid: Store at -70°C. Ship frozen on dry ice. Blood: Centrifuge blood, remove plasma, and freeze at -70C. Ship frozen on dry ice. CSF and plasma: Send to Viracor for HSV1 and HSV2 Real time qPCR #8500 Amniotic fluid: Send to Focus for Herpes Simplex Virus, Type 1 & 2 DNA, Quantitative Real-Time PCR # 43220 |
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| Units | copies/mL | ||||
| Normal range | Not detected | ||||
| Critical value | HSV from sterile sites | ||||
| Synonyms | HSV; PCR | ||||
| Stability | CSF and amniotic fluid: Frozen at -70C 1 month Plasma: Room temperature 4 days, frozen at -70C 1 month |
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| Turn around times | 2-5 days | ||||
| Additional information | HSV is a etiologic agent of encephalitis and aseptic meningitis as well as a wide variety of other infections. PCR for HSV in CNS disease is highly sensitive, whereas culture is often negative. This assay differentiates between HSV-1 and HSV-2. This test has not been cleared or approved by the U.S. Food and Drug Administration. It was developed and its performance characteristics have been determined by the performing laboratory. |
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| CPT coding | 87530-90 x2 | ||||
| LOINC code | 5014-6 | ||||
| Last Updated | 4/17/2013 2:28:53 PM | ||||
| Entry Number | 496 |
If you have additional questions regarding this test, please call: 415-353-1667