UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for Moffitt-Long and Mount Zion

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

Herpes simplex, DNA

Item Value
Approval req'd? Infants under 1 month of age require approval of Pediatric ID service.
Available Stat? No
Utilization Guidelines CSF testing should not be ordered on an immuncompetent patient unless there is no other explanation for CSF findings AND there are > 5 cells/mm3 or a protein of > 50 mg/dL These criteria do not apply if the patient is immunocompromised.
Test code Amniotic fluid: P319
Other samples: P337
Test group Herpes
Performed by Amniotic fluid: Focus Diagnostics
Other samples: Viracor
Sendout? Yes
Method Real time PCR
Container type
CSF or Amniotic fluid Sterile tube
Blood Lavender top
Amount to Collect
CSF or Amniotic fluid 1 mL
Blood 3 mL
Sample type CSF, amniotic fluid, EDTA plasma
Preferred volume
CSF or Amniotic fluid 1 mL
Plasma 1 mL
Min. Volume
CSF or Amniotic fluid 0.5 mL
Plasma 0.5 mL
Processing notes Test sent out and reported by Microbiology.

CSF and amniotic fluid: Store at -70°C. Ship frozen on dry ice.

Blood: Centrifuge blood, remove plasma, and freeze at -70C. Ship frozen on dry ice.

CSF and plasma: Send to Viracor for HSV1 and HSV2 Real time qPCR #8500

Amniotic fluid: Send to Focus for Herpes Simplex Virus, Type 1 & 2 DNA, Quantitative Real-Time PCR # 43220
Units copies/mL
Normal range Not detected
Critical value HSV from sterile sites
Synonyms HSV; PCR
Stability CSF and amniotic fluid: Frozen at -70C 1 month
Plasma: Room temperature 4 days, frozen at -70C 1 month
Turn around times 2-5 days
Additional information HSV is a etiologic agent of encephalitis and aseptic meningitis as well as a wide variety of other infections. PCR for HSV in CNS disease is highly sensitive, whereas culture is often negative. This assay differentiates between HSV-1 and HSV-2.

This test has not been cleared or approved by the U.S. Food and Drug Administration. It was developed and its performance characteristics have been determined by the performing laboratory.
CPT coding 87530-90 x2
LOINC code 5014-6
Last Updated 4/17/2013 2:28:53 PM
Entry Number 496

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center