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Herpes simplex, DNA
|Approval req'd?||Infants under 1 month of age require approval of Pediatric ID service.|
|Utilization Guidelines||CSF testing should not be ordered on an immuncompetent patient unless there is no other explanation for CSF findings AND there are > 5 cells/mm3 or a protein of > 50 mg/dL These criteria do not apply if the patient is immunocompromised.|
|Test code||Amniotic fluid: P319
Other samples: P337
|Performed by||Amniotic fluid: Focus Diagnostics
Other samples: Viracor
|Method||Real time PCR|
|Amount to Collect||
|Sample type||CSF, amniotic fluid, EDTA plasma|
|Processing notes||Test sent out and reported by Microbiology.
CSF and amniotic fluid: Store at -70°C. Ship frozen on dry ice.
Blood: Centrifuge blood, remove plasma, and freeze at -70C. Ship frozen on dry ice.
CSF and plasma: Send to Viracor for HSV1 and HSV2 Real time qPCR #8500
Amniotic fluid: Send to Focus for Herpes Simplex Virus, Type 1 & 2 DNA, Quantitative Real-Time PCR # 43220
|Normal range||Not detected|
|Critical value||HSV from sterile sites|
|Stability||CSF and amniotic fluid: Frozen at -70C 1 month
Plasma: Room temperature 4 days, frozen at -70C 1 month
|Turn around times||2-5 days|
|Additional information||HSV is a etiologic agent of encephalitis and aseptic meningitis as well as a wide variety of other infections. PCR for HSV in CNS disease is highly sensitive, whereas culture is often negative. This assay differentiates between HSV-1 and HSV-2.
This test has not been cleared or approved by the U.S. Food and Drug Administration. It was developed and its performance characteristics have been determined by the performing laboratory.
|CPT coding||87530-90 x2|
|Last Updated||4/17/2013 2:28:53 PM|