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Herpes simplex Culture
|Approval req'd?||Yes, contact Microbiology at x3-1268 for testing on urine from patients ≥ 1 month old, CSF, amniotic fluid or brochial lavage.|
|In House Availability||Set up daily, day and evening shifts|
|Collection Instructions||Using a cotton-tip swab with a wire shaft, rub vcessicle. Swab fluid of vesicle and rub base of lesion to obtain cells. Place swab in viral holding medium.
Swabs and viral holding medium are available from Microbiology
|Sample type||Vesicle, ulcer, corneal or conjunctival swab, tissue biopsy, vitreous fluid, urine, bronchial lavage, CSF, amniotic fluid|
|UCSF Rejection Criteria||Sample collected on Calgi-swabs. Swabs not received in viral holding media. Unsuitable sample types.|
|Synonyms||Viral culture; HSV|
|Stability||Room temperature 6 hours, refrigerated 1 day|
|Turn around times||5 days|
|Additional information||Includes typing positive cultures by DFA.
Herpes simplex DNA testing is more sensitive than culture for CSF and amniotic fluids.
Culture of the CSF for Herpes simplex will only be run in the setting of probable meningitis e.g. in disseminated infection in a newborn or for meningoencephalitis associated with genital herpes infection.
CSF culture will not be done in patients who present with an encephalitis only, for whom PCR is the appropriate test (see Herpes simplex, DNA).
|Last Updated||11/21/2013 12:52:44 PM|
If you have additional questions regarding this test, please call: 415-353-1667