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Herpes simplex Culture
|Approval req'd?||Yes, contact Microbiology at x3-1268 for testing on urine from patients ≥ 1 month old, bronchoalveolar lavage, vitreous fluid, amniotic fluid or CSF|
|In House Availability||Set up daily, day and evening shifts|
|Collection Instructions||Using a cotton-tip swab with a wire shaft, rub vcessicle. Swab fluid of vesicle and rub base of lesion to obtain cells. Place swab in viral holding medium.
Swabs and viral holding medium are available from Microbiology
|Amount to Collect||Tissue: 5 cu. mm, Urine and other fluids: 10 mL|
|Sample type||Tissue, bronchial, eye, genital swab, oral swab, urine, vesicle/ulcer swab, vitreous fluid, amniotic fluid, CSF|
|Preferred volume||Tissue: 5 cu. mm, Urine and other fluids: 10 mL
|Min. Volume||Tissue: 2 cu. mm, Urine and other fluids: 0.5 mL|
|UCSF Rejection Criteria||Sample collected on Calgi-swabs. Swabs not received in viral holding media. Unsuitable sample types.|
|Processing notes||If both DFA and culture ordered on a swab in VHM, give to Virology to prepare slide first.
HSV susceptibilities to acyclovir and/or foscarnet require approval.
|Synonyms||Viral culture; HSV|
|Stability||Room temperature 6 hours, refrigerated 1 day|
|Turn around times||5 days|
|Additional information||Typing by DFA is performed on positive cultures at an additional charge.
Herpes simplex virus DNA testing is more sensitive than culture for vitreous fluid, amniotic fluid, and CSF.
Culture of the CSF for Herpes simplex will only be run in the setting of probable meningitis e.g. in disseminated infection in a newborn or for meningoencephalitis associated with genital herpes infection.
CSF culture will not be done in patients who present with an encephalitis only, for whom PCR is the appropriate test (see Herpes simplex, DNA).
|Last Updated||7/28/2016 12:52:23 PM|