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Lab Manual for Moffitt-Long and Mount Zion

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Herpes simplex Antigen (DFA)

Item Value
Approval req'd? No
Available Stat? No
Test code P347
Test group Herpes
Performed by Microbiology
In House Availability Monday-Friday, day and evening shifts. Weekends, day shift only
Method Direct immunofluorescent assay
Collection Instructions Each slide should contain two spots of cells obtained from the base of an intact vesicle with a scalpel blade or swab. These spots should be approximately 1.5 cm in diameter (the size of a dime)
Container type Glass slide for smears, viral holding media for tissue biopsies
Sample type Tissue biopsy or smear of cells from base of vesicle (see collection instructions)
Preferred volume
Smear Two slides, each with two cell spots.


Tissue biopsy 1 gm
Min. Volume
Smear One slide with two spots or two slides each with one spot


Tissue biopsy 0.5 gm
UCSF Rejection Criteria Improperly collected sample. Unsuitable sample types.
Processing notes Prepare two touch prep smears from tissue biopsy.

Note: Do not reject samples submitted on swabs in holding media
Normal range Negative
Critical value Positive samples from inpatients and ED patients.
Synonyms HSV; HSV DFA; Herpes simplex Antigen; HSV Ag; HSV antigen
Stability Slides once prepared are stable at room temperature for 24 hours. For more prolonged stability, fix smears in acetone and freeze them at -70C.
Turn around times Same or next day
Additional information This test is NOT as sensitive as culture for Herpes simplex.
CPT coding 87300
LOINC code 5855-2
Last Updated 8/24/2011 12:23:37 PM
Entry Number 494
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