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Herpes simplex Antigen (DFA)

Item Value
Approval req'd? No
Available Stat? No
Test code P347
Test group Herpes
Performed by Microbiology
In House Availability Daily, day shift only
Method Direct immunofluorescent assay
Collection Instructions Each slide should contain two spots of cells obtained from the base of an intact vesicle with a scalpel blade or swab. These spots should be approximately 1.5 cm in diameter (the size of a dime). Allow material on slide to air dry.
Container type Glass slide for smears, viral holding media for tissue biopsies
Amount to Collect Vesicle/ulcer: Two slides, each with two cell spots, Tissue: 5 cu. mm
Sample type Tissue biopsy or smear of cells from base of vesicle (see collection instructions)
Preferred volume Vesicle/ulcer: Two slides, each with two cell spots, Tissue: 5 cu. mm
Min. Volume Vesicle/ulcer: One slide with two cell spots, Tissue: 2 cu. mm
UCSF Rejection Criteria Improperly collected sample. Unsuitable sample types.
Processing notes Prepare two touch prep smears from tissue. Make a fresh cut of tissue and touch it to the slides.
Give to Virology to make smears if swab in viral holding media is received.
Normal range Negative
Critical value Positive samples from inpatients and ED patients.
Synonyms HSV; HSV DFA; Herpes simplex Antigen; HSV Ag; HSV antigen
Stability Slides, once prepared, are stable at room temperature for 24 hours.

Specimens in viral holding media are stable at 2-8C until processed
Turn around times Same or next day
Additional information Sensitivity of the Herpes Simplex DFA test is approximately 80% as compared to culture.

Submit specimen for Herpes Culture if clinically indicated.
CPT coding 87300
LOINC code 5855-2
Last Updated 8/15/2016 5:25:38 PM
Entry Number 494
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