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Herpes simplex Antigen (DFA)

Item Value
Test Update Information Specimen sent to reference lab for testing due to reagent backorder by manufacturer. Result usually available in 48 to 72 hours.
Approval req'd? No
Available Stat? No
Test code P347
Test group Herpes
Performed by Microbiology
In House Availability Daily, day shift only
Method Direct immunofluorescent assay
Collection Instructions Each slide should contain two spots of cells obtained from the base of an intact vesicle with a scalpel blade or swab. These spots should be approximately 1.5 cm in diameter (the size of a dime)
Container type Glass slide for smears, viral holding media for tissue biopsies
Amount to Collect
Smear Two slides, each with two cell spots.

Tissue biopsy 3 cubic mm
Sample type Tissue biopsy or smear of cells from base of vesicle (see collection instructions)
Preferred volume
Smear Two slides, each with two cell spots.

Tissue biopsy 3 cubic mm
Min. Volume
Smear One slide with two spots or two slides each with one spot

Tissue biopsy 2 cubic mm
UCSF Rejection Criteria Improperly collected sample. Unsuitable sample types.
Processing notes Prepare two touch prep smears from tissue biopsy.

Note: Do not reject samples submitted on swabs in holding media
Normal range Negative
Critical value Positive samples from inpatients and ED patients.
Synonyms HSV; HSV DFA; Herpes simplex Antigen; HSV Ag; HSV antigen
Stability Slides, once prepared, are stable at room temperature for 24 hours.

Specimens in viral holding media are stable at 2-8C until processed
Turn around times Same or next day
Additional information Sensitivity of the Herpes Simplex DFA test is approximately 80% as compared to culture.

Submit specimen for Herpes Culture if clinically indicated.
CPT coding 87300
LOINC code 5855-2
Last Updated 10/1/2015 2:08:11 PM
Entry Number 494
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