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Herpes simplex Antigen (DFA)
|In House Availability||Daily, day shift only|
|Method||Direct immunofluorescent assay|
|Collection Instructions||Each slide should contain two spots of cells obtained from the base of an intact vesicle with a scalpel blade or swab. These spots should be approximately 1.5 cm in diameter (the size of a dime). Allow material on slide to air dry.|
|Container type||Glass slide for smears, viral holding media for tissue biopsies|
|Amount to Collect||Vesicle/ulcer: Two slides, each with two cell spots, Tissue: 5 cu. mm|
|Sample type||Tissue biopsy or smear of cells from base of vesicle (see collection instructions)|
|Preferred volume||Vesicle/ulcer: Two slides, each with two cell spots, Tissue: 5 cu. mm|
|Min. Volume||Vesicle/ulcer: One slide with two cell spots, Tissue: 2 cu. mm|
|UCSF Rejection Criteria||Improperly collected sample. Unsuitable sample types.|
|Processing notes||Prepare two touch prep smears from tissue. Make a fresh cut of tissue and touch it to the slides.
Give to Virology to make smears if swab in viral holding media is received.
|Critical value||Positive samples from inpatients and ED patients.|
|Synonyms||HSV; HSV DFA; Herpes simplex Antigen; HSV Ag; HSV antigen|
|Stability||Slides, once prepared, are stable at room temperature for 24 hours.
Specimens in viral holding media are stable at 2-8C until processed
|Turn around times||Same or next day|
|Additional information||Sensitivity of the Herpes Simplex DFA test is approximately 80% as compared to culture.
Submit specimen for Herpes Culture if clinically indicated.
|Last Updated||8/15/2016 5:25:38 PM|