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Lab Manual for Moffitt-Long and Mount Zion

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Hepatitis C Genotyping

Item Value
Approval req'd? No
Available Stat? No
Test code HCVG
Test group Hepatitis
Performed by Microbiology
In House Availability Every other Wednesday, evening shift
Method Multi-probe reverse hybridization (LiPA)
Container type Lavender top
Amount to Collect 4 mL blood
Sample type EDTA Plasma
Preferred volume 2 mL plasma
Min. Volume 0.5 mL plasma
UCSF Rejection Criteria Heparinized samples; Grossly hemolyzed samples; HCV viral load < 1000 copies/mL or no HCV viral load within 90 days of current specimen; Genotyping previously performed.
Processing notes Separate plasma from cells within 6 hours of collection and freeze at -20°C. Avoid repeated freezing and thawing of specimen.
Normal range
Synonyms HCV Genotyping
Turn around times Up to 16 days.
Additional information An interpretation of this test by a laboratory physician will automatically be performed and billed for.

In patients diagnosed with hepatitis C virus, genotyping is indicated for prognostic reasons. Patients with certain genotypes are more likely than others to respond to therapy with interferon and ribovirin.

Based on analysis of the sequences of the 5' untranslated regions and the core region of the HCV virus genome. There are six major genotypes (1-6) and multiple subtypes. Genotypes/subtypes reported with this assay are 1, 1a, 1b, 1a or 1b, 2, 2a or 2c, 2b, 3, 3a, 3b, 3c, 3k, 4, 4a/4c/4d, 4b, 4e, 4f, 4h, 5a, and 6a or 6b. Genotypes 1, 2 and 3 predominate in western countries and the far east, with types 2 and 3 more likely to respond to therapy with alpha interferon.

The assay requires a plasma viral RNA level of at least 1000 IU/mL. If a quantitative HCV test has not been done in the last 90 days, it will be ordered by the laboratory, reported and billed separately. The HCV genotyping order will be canceled/credited by the laboratory if the viral RNA level is < 1000 IU/ml. Consult microbiology if testing at a reference laboratory, by sequencing, is indicated.

This test was developed and its performance characteristics determined by the UCSF Clinical Labs. It has not been cleared or approved by the U.S. Food and Drug Administration.

Positive results are automatically forwarded to California public health per regulations. However, this does not absolve the ordering provider from any additional reporting if such is required.
CPT coding 87902, 83912
LOINC code 32286-7
LDT or Mod FDA? Yes
Last Updated 3/12/2014 8:53:27 AM
Entry Number 487

If you have additional questions regarding this test, please call: 415-353-1667

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