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Gram Stain

Item Value
Approval req'd? No
Available Stat? Sterile sites 24x7, Non-sterile sites 7:30 AM-11:30 PM
Test code P057
Performed by
Routine orders Microbiology
STAT orders Hematology at Parnassus


Note: Mount Zion STATs are performed by Microbiology at China Basin, except sterile site STATs are sent to Parnassus from 10:30 PM-7:30 AM.)
In House Availability Non-Stat requests performed daily 7:30 AM -11:30 PM

See information under 'Available stat?' for stat testing availability
Method Gram stain and Microscopy
Container type
CSF CSF tube or sterile tube
Fluids Sterile tube
Tissue Anaerobic transport medium or sterile container if large
Swabs Amies charcoal transport medium
Urine Urine cup or red-top vacutainer tube
Sputum: Clean container
Sample type Respiratory, urine*, genital, wound*, CSF**, body fluid**, unfixed tissue*

* considered 'sterile' if collected in the OR or by sterile means
** considered normally sterile sites

See Additional information
Normal range
Critical value Positive gram stains on samples from normally sterile sites (except urine) will be phoned.
Synonyms bacterial smear
Turn around times
STAT 1 hour
Routine Same or next day
Additional information Normally sterile site specimens include CSF and other sterile body fluids (e.g. vitreous, pleural, peritoneal, ascites, amniotic, or joint fluids, bile, dialysates), sterile body tissues, and other specimens from specialized invasive procedures (e.g. bronchoalveolar lavage), and transfused blood product in the case of a transfusion reaction.

For diagnosis of bacterial vaginosis or yeast infection on vaginal exudate, order Vaginal smear for Bacterial vaginosis/yeast.
CPT coding 87205
LOINC code 664-3
Last Updated 6/27/2014 1:19:23 PM
Entry Number 455
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