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Lab Manual for Moffitt-Long and Mount Zion

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Gram Stain

Item Value
Approval req'd? No
Available Stat? Parnassus:
Sterile sites 24x7
Non-sterile sites 7:30 AM - 11:30 PM


Mount Zion:
Sterile sites 7:30 AM - 10:30 PM (samples will be sent to Parnassus from 10:30 PM - 7:30 AM)
Non-sterile sites Samples sent to China Basin
Test code P057
Performed by Microbiology (Hematology for stats)
In House Availability Non-Stat requests performed daily 7:30 AM -11:30 PM

See information under 'Available stat?' for stat testing availability
Method Gram stain and Microscopy
Collection Instructions CSF: Submit in sterile tube in collection kit.

Fluids: Submit in a vacutainer tube (red top) or sterile black-top tube

Tissue: Submit in the clear anaerobic transport medium (OR) or in an empty sterile container.

Swabs: Always submit 2 swabs in the black Amies charcoal transport medium, one for the Gram stain and the other for the culture.

Urine: Submit in non-sterile clean, plastic cup or vacutainer tube without preservative (red top).

Sputum: Submit in non-sterile clean, plastic cup.

Bacterial vaginosis or yeast: Vaginal discharge (smear on a slide or swab in charcoal transport media) is to be submitted for Gram stain.

Note: for diagnosing GC from male urethra see: Gonococcus (GC only) Culture-male urethral specimens
Container type See Collection instructions
Sample type Respiratory, urine*, genital, wound*, CSF**, body fluid**, tissue*

* considered 'sterile' if collected in the OR or by sterile means
** considered normally sterile sites

See Additional information
Normal range
Critical value Positive gram stains on samples from normally sterile sites (except urine) will be phoned.
Synonyms bacterial smear
Turn around times
STAT 1 hour
Routine Same or next day
Additional information STAT Gram stains are read by Hematology at Moffitt-Long and by Mount Zion Laboratory.

STAT Gram stains are done on normally sterile site only.

Normally sterile site specimens include CSF and other sterile body fluids (e.g. vitreous, pleural, peritoneal, ascites, amniotic, or joint fluids, bile, dialysates), sterile body tissues, and other specimens from specialized invasive procedures (e.g. bronchoalveolar lavage), and transfused blood product in the case of a transfusion reaction
CPT coding 87205
LOINC code 664-3
Last Updated 9/8/2012 2:15:49 PM
Entry Number 455

If you have additional questions regarding this test, please call: 415-353-1667

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