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Free Hemoglobin, Plasma / Serum

Item Value
Approval req'd? No
Available Stat? Yes
Test code PHGBS
Performed by Parnassus Chemistry
Method HemoCue Plasma/Low Hb Photometer
Collection Instructions Results are highly dependent upon meticulous technique employed in collecting the specimen. To avoid hemolysis use an 18-19 ga. butterfly needle and discard the first 4 mL. Alternatively, apply a tourniquet and perform a venipuncture with an 18-19 ga. needle; collect any other tubes which are needed before releasing the tourniquet with the vacutainer still in the vein and collecting the sample for the hemoglobin assay. If no other samples are being collected at the same time, draw and discard one tube, release the tourniquet, then draw a second tube and submit the second tube for analysis.
Container type Light green top preferred, Gold top acceptable
Amount to Collect 2 mL blood
Sample type Heparinized plasma, serum or aqueous solutions
Preferred volume 1 mL serum, plasma, or aqueous solution
Min. Volume 0.2 mL serum, plasma, or aqueous solution
Units mg/dL
Normal range < 8 mg/dL
Synonyms hemoglobin, free; Free Hgb; Free Hb
Turn around times STAT 1 hour, Routine 4 hours
Additional information If extreme care is used to avoid hemolysis during phlebotomy, values of <8 mg/dL are expected. Note that this Hemocue assay has been modified to screen for levels of Plasma Free Hemoglobin > 8 mg/dL. If a more sensitive and precise assay for Plasma Free Hemoglobin is required, please order the sendout test for Plasma Free Hemoglobin (Quest test code FHGB).

This test is primarily intended to screen for hemolysis during extracorporeal membrane life support (ECLS) . In patients on ECLS, it is recommended that plasma hemoglobin levels > 50 mg/dL be investigated to determine whether it is being caused by a condition of the patient or by components of the ECLS circuit. See ELSO Guidelines for Cardiopulmonary Extracorporeal Life Support. Extracorporeal Life Support Organization, Version 1:1. April 2009, www.elso.med.umich.edu
To convert mg/dL to µmol/L (SI units) multiply by 0.155.
CPT coding 83051
LOINC code 721-1
Last Updated 6/21/2016 14:14:31
Entry Number 446
Lab Procedure Link Click here for procedure
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