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|Performed by||China Basin Chemistry|
|In House Availability||Set up Thursday, reports next day unless electrophoresis required|
|Method||HPLC (+/- Electrophoresis)|
|Collection Instructions||If collecting in citrate, check the expiration date on the label of the blue top vacutainer before drawing the patient|
|Container type||Lavender top, Citrate (blue) or Heparin (green) acceptable]|
|Amount to Collect||2 mL blood|
|Sample type||EDTA whole blood|
|Preferred volume||2 mL blood|
|Min. Volume||1 mL blood|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer.|
|Processing notes||Do not centrifuge.|
|Synonyms||Alkali denaturation; sickle hemoglobin; HbSS; Hemoglobin A2; Hemoglobin F; fetal hemoglobin; hemoglobin E; Hemoglobin C; hemoglobin electrophoresis; Hemoglobin Bart's; sickle screen; sickledex; HbC; HbS; HbE; Hgb S; Hgb C; HGB E|
|Turn around times||1-8 days|
|Reflex?||If variant hemoglobin is detected by HPLC, hemoglobin electrophoresis is performed to characterize the abnormality.|
|Additional information||Only A, A2 and F are normally present; the latter is almost completely replaced by A at age two. Hemoglobins A, A2, S, C, F, D or G, E or O, and fast hemoglobins, e.g., Hgb Bart's, are separated and quantitated.
Alkaline and/or citrate agar electrophoresis will be performed (at an additional charge) if abnormal hemoglobin(s) cannot be resolved by HPLC alone.
Once an abnormal hemoglobin has been confirmed by electrophoresis, all subsequent hemoglobinopathy requests that reveal the confirmed abnormal hemoglobin will be directly reported by the HPLC method.
HPLC is a rapid and reproducible method for identifying variants; however, it is possible that a rare variant may be missed. If clinical indications strongly suggest a variant hemoglobin not identified by HPLC, please contact the Chemistry Laboratory at 353-4820.
Iron deficiency decreases Hgb A2, and the characteristic elevation of Hgb A2 in beta-thalassemia may easily be missed.
Note: This test was developed by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. Food and Drug Administration.
|LDT or Mod FDA?||Yes|
|Last Updated||1/30/2013 12:01:54 PM|
If you have additional questions regarding this test, please call: 415-353-1667