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If you have additional questions regarding this test, please call: 415-353-1667
|Test Update Information||Effective 7/18/14, all hemoglobinopathy request will be tested by HPLC and Capillary Electrophoresis. Variant hemoglobins identified may need to be confirmed by electrophoresis (acid, alkaline, and/or fast).|
|Clinical Questions||Does my patient have thalassemia?|
|Performed by||China Basin Chemistry|
|In House Availability||Set up Thursday, reports next day unless electrophoresis required|
|Method||Capillary Electrophoresis (on Sebia Capillarys 2 Flex Piercing) and HPLC Electrophoresis (on Bio Rad Variant II) (+/- Electrophoresis)|
|Collection Instructions||If collecting in citrate, check the expiration date on the label of the blue top vacutainer before drawing the patient|
|Container type||Lavender top, Citrate (blue) or Heparin (green) acceptable]|
|Amount to Collect||2 mL blood|
|Sample type||EDTA whole blood|
|Preferred volume||2 mL blood|
|Min. Volume||1 mL blood|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer.|
|Processing notes||Do not centrifuge. Refrigerate samples|
Pediatric reference ranges adopted from Pediatric Reference Intervals 7th edition.
Adult reference ranges adopted from vendor (Sebia) performed studies and verified by UCSF Clinical Labs by running 52 outpatient samples with a normal RBC, WBC, Hct, MCV and A1c.
|Synonyms||Alkali denaturation; sickle hemoglobin; HbSS; Hemoglobin A2; Hemoglobin F; fetal hemoglobin; hemoglobin E; Hemoglobin C; hemoglobin electrophoresis; Hemoglobin Bart's; sickle screen; sickledex; HbC; HbS; HbE; Hgb S; Hgb C; HGB E|
|Stability||Refrigerated 1 week|
|Turn around times||1-8 days|
|Reflex?||If variant hemoglobin is detected by HPLC or capillary electrophoresis, hemoglobin electrophoresis may need to be performed to characterize the abnormality.|
|Additional information||Only A, A2 and F are normally present; the latter is almost completely replaced by A at age two. Hemoglobins A, A2, S, C, F, D or G, E or O, and fast hemoglobins, e.g., Hgb Bart's, are separated and quantitated.
Alkaline and/or citrate agar electrophoresis will be performed (at an additional charge) if abnormal hemoglobin(s) cannot be resolved by HPLC and capillary electrophoresis testing.
Once an abnormal hemoglobin has been confirmed, all subsequent hemoglobinopathy requests that reveal the confirmed abnormal hemoglobin will be directly reported by the capillary electrophoresis method.
HPLC and capillary electrophoresis are rapid and reproducible methods for identifying variants; however, it is possible that a rare variant may be missed. If clinical indications strongly suggest a variant hemoglobin not identified by HPLC & capillary electrophoresis, please contact the Chemistry Laboratory at 353-4820.
Iron deficiency decreases Hgb A2, and the characteristic elevation of Hgb A2 in beta-thalassemia may easily be missed.
Note: The HPLC test was developed by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. Food and Drug Administration.
|LDT or Mod FDA?||Yes|
|Last Updated||7/3/2014 3:10:03 PM|