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Fungal Culture, other fungi

Item Value
Clinical Questions Does my patient have histoplasmosis?
Approval req'd? Approval required for urine and joint fluid.
Available Stat? No
Test code P256
Test group Fungal Culture
Performed by Microbiology
In House Availability Set up daily, all shifts
Method Culture
Collection Instructions Swab specimens are not acceptable as they have very low fungal yields.
Container type
Body fluid, Tissue, BAL Sterile container
Sputum Clean container (urine cup)
Bone marrow Isolator tube (available from Hematology laboratory)
Amount to Collect
BAL 10-20 mL
Tissue 5 mm3
Urine 10-20 mL
Body fluid 5-10 mL
Sputum > 1 mL
Bone marrow 1 mL
Sample type Unfixed tissue, BAL, Sputum, Body fluid, Urine, Bone marrow
Preferred volume
BAL 10-20 mL
Tissue 5 mm3
Urine 10-20 mL
Body fluid 5-10 mL
Sputum > 1 mL
Bone marrow 1 mL
Min. Volume
Tissue 1-2 cu mm
BAL, Urine, Body fluid 2 mL
Sputum 1 mL
Bone marrow 0.5 mL
UCSF Rejection Criteria Samples on swabs, stool samples
Normal range No fungus isolated
Critical value KOH if C. immitis spherules present or non-septate hyphae suggestive of Zygomecetes; Biphasic fungus (e.g. Coccidiodes immitis) isolated. Zygomycete (e.g. mucormycosis) isolated. Positive cultures from CSF. Positive culture from a sample submitted for sterility testing
Synonyms H. capsulatum; Histoplasma capsulatum
Stability Refrigerated 24 hours
Turn around times Up to 30 days
Additional information Includes KOH-Calcofluor White Stain, except on urine and bone marrow.

For diagnosis of yeast and Cryptococcal meningitis, recommended tests are: CSF bacterial culture (yeast, including Cryptococcus neoformans grow well on routine bacterial culture) CSF and serum Cryptococcal Antigen (CrAg)

The yield of CSF cultures for other dimorphic fungi and invasive molds, including Aspergillus, Blastomyces, Histoplasma and the Zygomycetes (Mucor and Rhizopus) is extremely low. Tissue biopsy, if possible, is the recommended specimen for fungal diagnosis by culture.

CPT coding
Fungal culture 87102
KOH (If performed) 87206
LOINC code 580-1
Last Updated 7/31/2017 1:48:57 PM
Entry Number 404
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