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Fungal Culture, Blood
|Clinical Questions||Does my patient have histoplasmosis?|
|Approval req'd?||Yes, consult Microbiology at x3-1268 for fungal blood culture requests.|
|Utilization Guidelines||Fungal blood cultures are indicated only for Histoplasma capsulatum, however, bone marrow is the preferred specimen for this organism.
Yeasts such as candida grow well in routine blood cultures and a specific 'fungal culture' order is unnecessary for detecting yeasts.
Aspergillus and other molds will not grow in blood cultures; a tissue specimen is required for these organisms.
|Test group||Fungal Culture|
|In House Availability||Set up daily, day and evening shifts|
|Container type||Dark green top x 2 (DO NOT use Lithium heparin i.e. Light Green top tube.)|
|Amount to Collect||8 mL blood|
|Sample type||Heparinized blood|
|Preferred volume||8 mL blood|
|Min. Volume||4 mL blood|
|UCSF Rejection Criteria||Blood submitted in Lithium heparin (Lt. Green top) vacutainer.|
|Critical value||Biphasic fungus (e.g. Coccidiodes immitis) isolated.|
|Synonyms||H. capsulatum; Histoplasma capsulatum|
|Stability||Refrigerated 24 hours|
|Turn around times||Up to 4 weeks|
|Additional information||Routine blood cultures are incubated for 5 days, sufficient to detect yeast.
Differentiation of Candida dublinensis from the biochemically and clinically similar species Candida albicans is performed on request only.
|Last Updated||12/19/2013 9:35:37 AM|