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Lab Manual for Moffitt-Long and Mount Zion

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Fungal Culture, Blood or Bone marrow

Item Value
Approval req'd? Yes, consult Microbiology at x3-1268 for fungal blood culture requests.
Available Stat? No
Utilization Guidelines Fungal blood cultures are indicated only for Histoplasma capsulatum, however, bone marrow is the preferred specimen for this organism.

Yeasts such as candida grow well in routine blood cultures and a specific 'fungal culture' order is unnecessary for detecting yeasts.

Aspergillus and other molds will not grow in blood cultures; a tissue specimen is required for these organisms.
Test code P258
Test group Fungal Culture
Performed by Microbiology
In House Availability Set up daily, day and evening shifts
Container type
Blood: Dark green top x 2 (DO NOT use Lithium heparin i.e. Light Green top tube.)
Bone marrow: Isolator tube (available from Hematology)
Amount to Collect
Blood: 8 mL
Bone marrow: 1.5 mL
Sample type Heparinized blood or bone marrow
Preferred volume
Blood: 8 mL
Bone marrow: 1.5 mL
Min. Volume
Blood: 4 mL
Bone marrow: 1 mL
UCSF Rejection Criteria Blood submitted in Lithium heparin (Lt. Green top) vacutainer.
Normal range
Critical value Biphasic fungus (e.g. Coccidiodes immitis) isolated.
Synonyms H. capsulatum; Histoplasma capsulatum
Stability Refrigerated 24 hours
Turn around times Up to 4 weeks
Additional information Routine blood cultures are incubated for 5 days, sufficient to detect yeast.

If a history of travel to an endemic area suggests the need to culture for histoplasma or other agents, contact Microbiology (x3-1268). Submit bone marrow in a 1.5 mL ISOLATOR tube (ask that the Hematology technologist bring a tube to the bone marrow collection procedure), and specify, e.g., "Look for Histoplasma" on the request slip. KOH smears are not routinely performed on bone marrow.

See also Mycology section in text at front of Lab Manual.
CPT coding 87103
LOINC code 572-8
Last Updated 5/19/2011 3:36:23 PM
Entry Number 402

If you have additional questions regarding this test, please call: 415-353-1667

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