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Fetal Cells, Quantitative
|Approval req'd?||Yes, contact Hematology (Parnassus: 3-1747, Mission Bay: call 6-0194). If requesting testing on adult females of child-bearing age.|
|Performed by||Parnassus & Mission Bay Hematology|
|In House Availability||Test run 0800-1500 Monday-Friday|
|Method||Modified Acid Elution as described by Kleihauer-Betke and modified by Clayton.|
|Collection Instructions||Complete appropriate requisition and request "Fetal cells, Quantitative"|
|Container type||Lavender top, CSF tube or sterile collection tube|
|Amount to Collect||1 mL blood|
|Sample type||EDTA whole blood, Body Fluid, CSF|
|Preferred volume||1 mL blood, Body fluid or CSF|
|Processing notes||If test cannot be performed immediately refrigerate sample.|
|Synonyms||Kleihauer-Betke Test; Acid elution test; Alkali Denaturation; Betke test; fetal RBC's; Kleihauer test|
|Stability||Blood refrigerated 48 hours. CSF and Body fluids refrigerated 4-6 hours.|
|Turn around times||Same day or next weekday.|
|Additional information||Testing for a feto-maternal bleed will NOT be done in the case of an Rh+ infant born to an Rh- mother if the more sensitive Fetal Bleed Screen (rosetting test) run by the Blood Bank is negative.
Other anticoagulants or clotted blood may be used if necessary. A blood sample must be submitted at the same time and tested in parallel with CSF or BF. Each specimen examined is charged separately.
Brecher M, ed. Technical Manual, 15th ed. Bethesda, Maryland: American Association of Blood Banks, 2005.
Petrides, M. Practical Guide to Transfusion Medicine, 2nd. Bethesda, Maryland: AABB Press, 2007
|Last Updated||9/30/2015 3:18:56 PM|