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Lab Manual for Moffitt-Long and Mount Zion

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E. coli O157 culture

Item Value
Approval req'd? No
Available Stat? No
Test code P157
Performed by Microbiology
In House Availability Set up daily, day and evening shifts.
Method Culture
Collection Instructions Submit stool to laboratory within 3 hours of collection.

If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary-Blair) Medium collection vial. Add stool to red line on vial and mix well with spoon. Obtain vials from outpatient phlebotomy stations or from the Microbiology Lab.

Two specimens collected 24-48 hours apart are sufficient for the detection of the majority of pathogens.
Container type Urine cup or Cary Blair transport medium
Amount to Collect 5 mL
Sample type Stool
Preferred volume 5 mL
Min. Volume 5 mL
UCSF Rejection Criteria Unpreserved stool received > 3 hours after collection. More than two samples per episode or more than one sample received within 24 hours.
Normal range
Critical value Positive for E. coli O157
Synonyms Shiga toxin; Toxigenic E. coli; Enteropathogenic E coli
Stability Unpreserved 3 hours, preserved 1 week
Turn around times 1-3 days
Additional information If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed if appropriate.

Susceptibility testing may be omitted upon request when sample is submitted for testing.

Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.
CPT coding 87046
LOINC code 10851-4
Last Updated 1/14/2014 8:49:43 AM
Entry Number 328
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