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Lab Manual for Moffitt-Long and Mount Zion

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White Blood Cell Differential

Item Value
Approval req'd? No
Available Stat? Yes
Test code CBCD, WBCDF
Performed by Parnassus & Mt. Zion Hematology
In House Availability Test available 24 hours per day 7 days per week
Container type Lavender top
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
Min. Volume 1 mL blood (or 250 µL in a pedi-bullet)
Units x109/L
Normal range Normal Absolute Counts in x109/L (95% confidence interval):

0-24 hours 5.0-28 2.0-12 0.4-3.1 0.0-0.9 0.0-0.6
24 hours-1 wk 1.5-21 2.0-17 0.3-2.7 0.1-1.1 0.0-0.3
1 wk-6 months 1.0-10 2.0-17 0.2-2.7 0.1-1.1 0.0-0.3
6 months -4 years 1.0-8.5 2.0-14 0.0-0.9 0.0-1.1 0.0-0.3
4-14 years 1.5-8.5 1.2-8.0 0.0-1.4 0.0-1.1 0.0-0.3
14-21 years 1.8-8.0 1.0-6.1 0.0-1.4 0.0-0.8 0.0-0.3
> 21 years 1.8-6.8 1.0-3.4 0.2-0.8 0.0-0.4 0.0-0.1
Critical value Neutrophils ≤ 1.0 x109/L

Note: Neutrophil counts ≤ 1.0 x109/L are not called if a prior critical was reported in the preceding 24 hours
Synonyms Eosnophil; Basophil; Neutrophiil; Granulocyte; Monocyte; Lymphocyte; Leukocyte differential; WBC differential
Turn around times Stat 1 hour, Routine 4 hours
Additional information All differential counts are routinely performed by automated flow cytometry.

Total neutrophils, both mature PMNs and immature forms, are counted. The presence of immature granulocytes/left shift (metamyelocytes and myelocytes) is reported, but not quantitated. Smears with this finding will be reviewed microscopically if a left shift has not already been noted within the previous 72 hours.

A manual differential will be initiated by the laboratory at a separate charge if an automated differential is technically inadequate for the analysis of a particular sample.

Manual differentials also include RBC morphology and platelet morphology.
Last Updated 6/7/2013 10:50:26 AM
Entry Number 305

If you have additional questions regarding this test, please call: 415-353-1667

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