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Lab Manual for Moffitt-Long and Mount Zion

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Cutaneous Antibodies

Item Value
Approval req'd? No
Available Stat? No
Test code No test code, see Processing instructions
Performed by UCSF IF Lab
Sendout? Yes
In House Availability Daily, Monday-Friday
Method Indirect immunofluorescence
Collection Instructions Complete Dermatopathology requisition and submit with sample. Samples should be received by the laboratory by Wednesday for next Monday signout.
Container type Gold top
Amount to Collect 4 mL blood
Sample type Serum
Preferred volume 2 mL serum
Min. Volume 1 mL serum
Processing notes Store and transport at room temperature. Send sample and completed dermatopathology requisition to: UCSF Dermatopathology office, Mount Zion Campus, 1701 Divisadero St., 3rd floor Room 350, San Francisco, CA 94115.
For questions contact the UCSF Immunofluorescence Lab at 353-7546.
Units titer
Normal range Negative titer < 1:10
Synonyms Blistering diseases; blistering disorders; BMZ antibodies; Bullous disease; Cutaneous immunofluorescent antibodies; basement membrane antibodies; epidermolysis bullosa aquisita; fluorescent antibodies for bullous disease; Pemphigoid; pemphigus
Turn around times 3-5 days
Additional information Identifies the presence of serum antibodies against intercellular substance in squamous epithelium (pemphigus vulgaris) or basement membrane (bullous pemphigoid). Nuclear staining may be seen from sera of patients with systemic lupus or other connective tissue disorders. Antibodies are typically present
during active disease. If positive, it is advisable to perform direct immunofluorescent testing on biopsies of skin lesions. A two-fold drop in titer is indicative of effective therapy.
Last Updated 5/22/2013 2:12:25 PM
Entry Number 281
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