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Cortisol, serum

Item Value
Test Update Information Method changed from Siemens Centaur XP platform to Abbott Architect i1000 platform on October 3, 2016. The Abbott Architect cortisol assay has less cross reactivity with synthetic and endogenous steroids than the previous Siemens Centaur assay and correlates better with mass spectrometry cortisol assays.
Approval req'd? No
Available Stat? No
Test code CORT
Test group Cortisol
Performed by China Basin Chemistry
In House Availability Test available on day shift, 7 days per week.
Method Chemiluminescent Microparticle Immunoassay (Abbott Architect i1000)
Collection Instructions A morning sample is usually recommended.
Container type Preferred: Gold top or Red top; Acceptable: Green Top (Sodium/Lithium Heparin)
Amount to Collect 1 mL blood
Sample type Serum
Preferred volume 0.5 mL serum
Min. Volume 0.2 mL serum
UCSF Rejection Criteria Samples will not be rejected based on time of collection.
Processing notes Refrigerate
Units µg/dL
Normal range Adult Reference Range

AM (before 10am) 4 – 19 ug/dL
PM (after 5pm) 3 – 17 ug/dL

Reference range adopted from vendor performed studies and verified by running lab personnel.

Pediatric Reference Range

Age Range
2 - 14 days 0.5 – 12
15 days – < 1 year 0.5 – 17
1 year - < 9 years 2 – 11
9 - < 14 years 2 – 13
14 - < 17 years 3 – 16
17 years – < 19 years 4 – 18

Pediatric reference ranges adopted from CALIPER Pediatric Reference Interval study performed on random samples using the Abbott Architect i2000 assay. Morning samples gave results approximately 10% higher than afternoon samples.
Bailey et al. Clinical Chemistry 59:9 1393–1405 (2013)
Synonyms Compound F; 11-hydroxycorticosteroids; 11-hydroxycorticoids; 11-OH steroids; 11-OH corticosteroids; Cosyntropin provocative testing
Turn around times 1-3 days
Additional information The Architect cortisol assay is highly specific for cortisol and correlates well with mass spectrometry methods (Dodd AJ et al. Annals of Clinical Biochemistry 2014).

The specificity of the ARCHITECT Cortisol assay was determined by studying the cross-reactivity of compounds whose chemical structure or concurrent usage may potentially interfere with the ARCHITECT Cortisol assay. Specificity of the assay was determined by spiking each compound into human serum specimens with cortisol levels of 11.4 and 12.0 µg/dL. Cross reactivity results with other steroids are listed below.

Compound Conc (ug/dL) % Cross-Reactivity
Aldosterone 1000 0.0
Beclomethasone 1000 0.0
Budesonide 1000 0.0
Canrenone 1000 0.1
Corticosterone 1000 0.9
Cortisol 21-glucuronide 1000 0.2
Cortisone 1000 2.7
B-Cortol 1000 0.0
B-Cortolone 1000 0.0
11-Deoxycorticosterone 100 0.0
11-Deoxycortisol 100 1.9
Dexamethasone 1000 0.0
DHEA 1000 0.0
DHEA-S 1000 0.0
B-Estradiol 1000 0.0
Estriol 1000 0.0
Estrone 1000 0.0
Fludrocortisone 100 36.6
Fluticasone Propionate 1000 0.0
6B-Hydroxycortisol 1000 0.2
17a-Hydroxypregnenolone 1000 0.1
11B-Hydroxyprogesterone 1000 0.2
17-Hydroxyprogesterone 1000 0.6
Medroxyprogesterone Acetate 1000 0.0
6-Methylprednisolone 1000 0.1
Mometasone 1000 0.0
Prednisolone 100 12.3
Prednisone 1000 0.0
Pregnanetriol 1000 0.0
Pregnenolone 1000 0.0
Progesterone 1000 0.0
B-Sitosterol 1000 0.0
Spironolactone 1000 0.0
Testosterone 1000 0.0



CPT coding 82533
LOINC code 2143-6
Last Updated 10/7/2016 1:14:18 PM
Entry Number 258
Lab Procedure Link Click here for Procedure
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