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Cortisol, serum

Item Value
Test Update Information Effective June 2, 2010, the Cortisol values will read approximately 10% lower due to a restandardization of the calibrator materials from the reagent vendor. This new restandardization will bring results closer to Gas Chromatography-Mass Spectrometry (GC-MS) values.
Approval req'd? No
Available Stat? No
Test code CORT
Test group Cortisol
Performed by China Basin Chemistry
In House Availability Test available on day shift, 7 days per week.
Method Chemiluminescent immunoassay (Siemens Centaur)
Collection Instructions Morning samples (AM) should be drawn between 0700 and 0900 hours. Afternoon (PM) samples should be drawn between 1500 and 1700 hours. Normal ranges are based upon collection of samples within these timeframes.
Container type Gold top or Red top
Amount to Collect 1 mL blood
Sample type Serum
Preferred volume 0.5 mL serum
Min. Volume 0.2 mL serum
UCSF Rejection Criteria Samples collected outside of stated time frames
Processing notes Refrigerate
Units µg/dL
Normal range
AM (0700-0900 hours) 4-22 µg/dL
PM (1500-1700 hours) 3-17 µg/dL


Reference range adopted from vendor performed studies and verified by running lab personnel
Synonyms Compound F; 11-hydroxycorticosteroids; 11-hydroxycorticoids; 11-OH steroids; 11-OH corticosteroids; Cosyntropin provocative testing
Turn around times 1-3 days
Additional information The Centaur Cortisol assay is highly specific for cortisol.

Cross-reactivity by structurally related compounds and pharmaceuticals was determined by spiking each compound into separate human serum samples to a final level of 1000 µg/dL (27,590 nmol/L), unless otherwise noted.

% cross-reactivity = ((cortisol in spiked sample, µg/dL – cortisol in unspiked sample, µg/dL) x 100) / (concentration of compound added, µg/dL)

Compound % Cross-Reactivity
Aldosterone 0.4
Allotetrahydrocortisol (100 ug/dL) 11.9
Androstenedione 0.2
Corticosterone 2.6
Cortisone (100 ug/dL) 11.5
α-cortol 0.6
α-cortolone 0.1
β-cortol 0.1
β-cortolone ND
Dehydrocorticosterone 2.7
11-deoxycorticosterone 0.9
11-deoxycortisol (100 ug/dL) 18.3
21-deoxycortisol (100 ug/dL) 10.3
20α-dihydrocortisol 2.5
20β-dihydrocortisol 2.5
20α-dihydrocortisone 0.5
20β-dihydrocortisone 0.3
11β-hydroxyandrosterone ND
6β-hydroxycortisol 2.3
11β-hydroxyetiocholanolone ND
11β-hydroxyprogesterone 1.0
17α-hydroxyprogesterone 1.4
17β-hydroxypregnanolone 0.1
11-keto-androsterone ND
11-keto-etiocholanolone ND
Pregnanetriol ND
Pregnenolone 0.1
Progesterone 0.5
Spironolactone 0.1
Testosterone 0.3
Tetrahydrocortisol 1.1
Tetrahydrocortisone 0.5
Tetrahydro-11-deoxycortisol 0.7
Synthetic steriods
Prednisolone (50 ug/dL) 92
6-methyl-prednisolone (100 ug/dL) 23.1
Dexamethasone 0.5
Prednisone (100 ug/dL) 10.7
Canrenone 0.2



* ND = Not Detectable
CPT coding 82533
LOINC code 2143-6
Last Updated 5/29/2016 9:48:33 PM
Entry Number 258
Lab Procedure Link Click here for Procedure
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