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Item Value
Approval req'd? No
Available Stat? No
Test code AMIKP (Peak), AMIKT (trough), AMIKRN (random)
Performed by UC Irvine
Sendout? Yes
Method Enzyme-Multiple immunoassay Technique (EMIT)
Collection Instructions Time to Steady State: 2-3 doses (1 dose for ICN extended interval dosing)

Trough samples should be collected 30 minutes before 3rd or 4th dose. Document exact time of collection in Apex, on requisition AND sample label.

Draw peak sample for peak 30 min. after end of infusion or 60 minutes after IM dose.
Container type Red top (Gold top NOT acceptable)
Amount to Collect 2 mL blood
Sample type Serum
Preferred volume 1 mL serum
Min. Volume 0.5 mL
UCSF Rejection Criteria Collected in Gold top
Processing notes Freeze sample and ship frozen.
Units mg/L
Normal range
Peak 20.0 - 30.0 µg/mL
Trough < 8.0 µg/mL
Critical value UC Irvine has critical values for Amikacin:

Age Amikacin Critical value
< 12 months > 30.0 µg/mL
≥ 12 months > 35.0 µg/mL

Age Amikacin level
< 12 months ≥ 10.0 µg/mL
≥ 12 months ≥ 8.0 µg/mL
Stability Room temperature 4 hours, refrigerated 1 week, frozen 1 month.
Turn around times Test run daily. Turnaround time: 1-2 days.
Additional information Concurrent kanamycin treatment interferes with assay.

Click here for: A Guide on Drug Level Monitoring
CPT coding 80150-90
Last Updated 11/16/2014 7:06:29 PM
Entry Number 233
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