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Lab Manual for Moffitt-Long and Mount Zion

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Chromosome Analysis, Tissue (Reactivated)

Item Value
Approval req'd? No
Available Stat? No
Test code CYTIS
Test group Chromosome Analysis
Performed by Medical Genomics - Cytogenetics
In House Availability Set up daily, Monday-Friday
Method Giemsa banding and brightfield microscopy
Collection Instructions Submit sample in a sterile screw-top container filled with Tissue culture transport media. If Cytogenetics tisue transport media is not available RPMI, Hanks or sterile saline is aceptable.
Container type 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect 30 mg tissue
Sample type Unfixed Tissue (including POC)
Preferred volume 30 mg tissue
Min. Volume 5 mg tissue
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples; samples in formalin.
Processing notes Maintain sample at room temperature. Transport to CB Cytogenetics within 24 hours.
Normal range 46,XY normal male
46,XX normal female
Synonyms Cytogenetic analysis; Karyotype; Karyotyping; Tissue cytogenetics; products of conception; POC
Stability Room temperature 1 day, refrigerated 1 day, frozen unacceptable.
Turn around times 14-28 days
Reflex? If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
CPT coding 88262, 88233
LOINC code 48818-9
Last Updated 1/22/2014 8:57:04 AM
Entry Number 205

If you have additional questions regarding this test, please call: 415-353-1667

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