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Lab Manual for Moffitt-Long and Mount Zion

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Chromosome Analysis, Chorionic Villi

Item Value
Approval req'd? No
Available Stat? No
Test code CYCV
Test group Chromosome Analysis
Performed by Medical Genomics - Cytogenetics
In House Availability Set up daily, Monday-Friday
Method Giemsa banding and brightfield microscopy
Collection Instructions Collect 25 mg and distribute into two 15 mL orange screw top polypropylene tubes that are filled with transport media. Discard and remove visible maternal deciduas prior to collecting in transport tubes.

Complete and submit a "UCSF Reproductive Genetics Lab Results" or a "UCSF Cytogenetic Requisition" form and send with the samples.

Click here for Cytogenetics Requisition

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason.
Container type 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect 25 mg chorionic villi
Sample type CVS
Preferred volume 25 mg chorionic villi
Min. Volume 5 mg chorionic villi
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Normal range 46,XY normal male
46,XX normal female
Synonyms Cytogenetic analysis; Prenatal Cytogenetics; Chorionic villi; CVS; Karyotype; Karyotyping
Turn around times 7-14 days
Reflex? If an abnormality is detected ta Cytogenetics Director will determine the appropriate additional studies (e.g. C-banding, NOR) to be performed to characterize the abnormality.

If additional metaphases are required for final interpretation additional counts will be performed and billed for.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
Additional information Minimum volume for an optimal result is greater than or equal to 5 mg. Samples less than 5 mg may result in inconclusive results and a repeat sample may be requested.
CPT coding 88235, 88267, 88280
LOINC code 48818-9
Last Updated 1/22/2014 8:57:02 AM
Entry Number 203

If you have additional questions regarding this test, please call: 415-353-1667

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