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Lab Manual for Moffitt-Long and Mount Zion

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Chromosome Analysis, Amniotic Fluid (includes Alpha-fetoprotein, amniotic fluid)

Item Value
Approval req'd? No
Available Stat? No
Test code CYAFB
Test group Chromosome Analysis
Performed by Medical Genomics - Cytogenetics & China Basin Chemistry
In House Availability Set up daily, Monday-Friday
Method Giemsa banding and brightfield microscopy
Collection Instructions Sample is to be collected in two 15 mL orange screw top polypropylene tubes. Discard first 2 mL of fluid, and then add 15-25 mL to two tubes.

If the sample is grossly bloody, or the sample volume is < 10 mL the results may be inconclusive and a repeat sample will be requested.

Complete and submit a "UCSF Reproductive Genetics Lab Results" or a "UCSF Cytogenetic Requisition" form and send with the samples.

Click here for Cytogenetics Requisition

Keep samples at Room temperature. DO NOT centrifuge.
Container type Sterile centrifuge tube with screw cap, polypropylene preferred.
Amount to Collect 30 mL amniotic fluid
Sample type Amniotic fluid
Preferred volume 30 mL amniotic fluid
Min. Volume 10 mL amniotic fluid
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes This package code orders CYAF, AFPX and SENDO.

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Normal range 46,XY normal male
46,XX normal female
Synonyms Cytogenetic analysis; Prenatal cytogenetics; Amniotic fluid; Karyotype; Karyotyping
Stability 48 hours
Turn around times 7-14 days
Reflex? If an abnormality is detected ta Cytogenetics Director will determine the appropriate additional studies (e.g. C-banding, NOR) to be performed to characterize the abnormality.

If additional metaphases are required for final interpretation additional counts will be performed and billed for.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
CPT coding 88235, 88267, 88280, 82106
LOINC code 48818-9
Last Updated 1/22/2014 8:57:02 AM
Entry Number 202
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