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Chlamydia Culture

Item Value
Approval req'd? No
Available Stat? No
Test code P699
Test group Chlamydia
Performed by Focus Diagnostics
Sendout? Yes
Method Tissue Culture, IFA
Patient Preparation Some spermicidal agents and feminine powder sprays interfere with Chlamydia testing and should not be used prior to specimen collection.
Collection Instructions Do not use wood swabs. Dacron or rayon on plastic or wire swabs are recommended for specimen collection. Specimen should contain columnar or cuboidal epithelial cells from the infected site.

Collection kits with Universal Transport Medium (UTM) can be obtained from Moffit-Long Laboratory or Mount Zion Laboratory. Break or bend swabs to fit inside UTM tube. Immerse biopsy in UTM tube
Container type
Swabs and tissue Universal Transport Medium (UTM)
Other specimens Sterile container
Amount to Collect
Swab samples One swab
Fluids 3 mL
Tissue 1 cu mm
Sample type Endocervical swab, urethral swab, conjunctival eye scraping (swab), rectal swab, lymph node aspirate/biopsy, biopsy, vaginal swab (<12 years old), nasopharyngeal swab (infants only), throat swab, and endotracheal aspirate (infants only)
Preferred volume
Swab samples One swab
Fluids 3 mL
Tissue 1 cu mm
Min. Volume
Swab samples One swab
Fluids 1 mL
Tissue 1 cu mm
UCSF Rejection Criteria Collected with wooden handle swab
Processing notes If fluid, transfer 3 ml to UTM media or 1 part fluid to 1 part UTM media. Transport specimens in UTM to China Basin using cold packs. Freeze specimen at -70C upon receipt at China Basin, and ship frozen to reference lab. Focus test code 690.
Normal range No Chlamydia isolated
Synonyms C. pneumoniae; C. psittaci; C. trachomatis; TWAR agent; Inclusion conjunctivitis; Trachoma; TRIC; congenital infection; prenatal infection
Turn around times 3-7 days.
Additional information Nucleic acid detection is a sensitive method for diagnosis of CT / NG infections and is the recommended method for most patients. Culture for Chlamydia trachomatis can be ordered to assess clinical treatment failure (where DNA may persist post-treatment), cases of suspected sexual abuse, or for sample types other than cervical, urethral, rectal, pharyngeal, vaginal and urine (e.g. eye swab, tissue, extra-genital infection sites).

Chlamydia trachomatis is identified through the use of monoclonal antibodies specific for the major outer membrane protein present in all 15 known serovars of C. trachomatis but not C. pneumoniae or C. psittaci. Contact Microbiology if testing for the latter two species are clinically indicated. Substances that may interfere with growth or isolation of Chlamydia include antibiotics administered to the patient prior to specimen collection.
CPT coding 87110-90, 87140-90
LOINC code 560-3
Last Updated 11/19/2013 12:51:26 PM
Entry Number 182
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