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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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mNGS Pathogen Dx

Item Value
Approval req'd? Yes, test requires approval by Microbiology for UCSF patients. Requests from outside UCSF require an institutional account prior to sample submission.

In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information').
Available Stat? No
Utilization Guidelines Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
Test code P710
Performed by Microbiology
In House Availability Yes, upon consultation with the Infectious Diseases or Neurology service for UCSF patients.
Collection Instructions Lumbar puncture
Container type CSF tube or sterile black-top tube
Sample type CSF
Preferred volume 2ml
Min. Volume 1ml
UCSF Rejection Criteria Samples submitted in incorrect tube, grossly hemolyzed samples
Processing notes UCSF: Freeze at -70°C on receipt at China Basin.
Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.

With approval from the Microbiology Laboratory, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius Collection and Processing instructions click here
Normal range No microorganisms detected
Synonyms Metagenomic next-generation sequencing, deep sequencing
Stability Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25 C or -70 C for 1 month
Turn around times 1-2 weeks
Additional information With approval from the Microbiology Laboratory, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius Collection and Processing instructions click here
CPT coding 81479
LDT or Mod FDA? Yes
Last Updated 12/28/2017 10:50:38 AM
Entry Number 1733
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