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Acanthamoeba Culture and smear

Item Value
Available Stat? No
Test code P417
Test group Parasitology
Performed by Microbiology
In House Availability Monday-Friday day shift
Method Culture, and exam of smear by microscopy
Collection Instructions Transport specimens to Microbiology as soon as possible.

Corneal scrapings or biopsy: Place specimen directly onto the center of a non-nutrient agar plate. Label the plates at the edge so as not to interfere with microscopic analysis. Tape the plate closed and transport in a sealed specimen bag. If a stain is requested, submit a slide with a centered dime-sized smear, frosted side face up, labeled, and in a slide transport container.

Contact lens solution: Submit in original container or sterile container.

Contact lens case: Submit with fluid or with lenses.
Container type Corneal scraping/biopsy: Non-nutrient agar plate, Contact lens solution: Sterile container, Contact lens case with solution or lens
CSF/Tissue: CSF tube or sterile collection tube
Amount to Collect Contact lens solution: 0.5 mL
CSF: 5 mL
Tissue: approximately 6 mm
Sample type Corneal scraping/biopsy
Contact lens solution
Contact lens case with fluid or lens
Brain tissue
Skin abscess
Preferred volume Contact lens solution: 0.5 mL
CSF: 5 mL
Tissue: 6 mm
Min. Volume Contact lens solution: 0.5 mL
CSF: 2 mL
Tissue: 3 mL
UCSF Rejection Criteria Dry contact lens case without contact lens
Processing notes Store sample at room temperature.
Notify a parasitologist or CLS when specimen arrives.
Normal range No Acanthamoeba spp. present/isolated
No Parasites Seen
Synonyms Acanthamoeba, Naegleria, Balamuthia, Amoeba
Turn around times Culture: 7-10 days, Smear: 1-3 days
Additional information A culture for Acanthamoeba will be performed on all specimen types.

An additional charge is billed for the smear.

For ophthalmology specimens, a smear is stained and examined when a slide is received, and when contact lens solution or contact lens case with fluid or lens is received.
CPT coding Culture: 87081, Smear:87207
Last Updated 6/5/2017 9:52:45 AM
Entry Number 1722
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