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Bacterial Culture, Normally Sterile Site, with Gram stain (New test)

Item Value
Approval req'd? No
Available Stat? No
Test code P095
Test group Bacterial culture
Performed by Microbiology
In House Availability Set up daily, day and evening shifts
Method Aerobic and anaerobic culture
Gram stain
Collection Instructions Submitting peritoneal fluid
(abdominal fluid, ascitic fluid, dialysis fluid) for bacterial culture:

1. inoculate 10 ml into each of aerobic and anaerobic blood culture bottles at the patient's bedside for optimal growth and recovery of organisms. Divide the fluid equally among the bottles if < 20 mL is obtained.

2. Additional fluid may be submitted in a sterile tube, which allows for gram stain to be performed and faster identification of bacteria present at high concentrations.

Submitting tissue
For tissues not collected in the OR, obtain anaerobic transport medium from Microbiology L553 or from the Mount Zion OR

Submitting bone marrow aspirate
Schedule in advance at the time the bone marrow is requested.
Container type
Peritoneal fluid Aerobic and anaerobic blood culture bottles, and sterile tube
Non-peritoneal fluid Sterile tube
Tissue Anaerobic transport vial or sterile container
Bone marrow aspirate 1.5 mL Isolator tube (available from Microbiology L553 or Hematology M524)
Swabs Amies transport medium with charcoal
Amount to Collect
Peritoneal fluid 20 mL
Non-peritoneal fluid 25 mL
Tissue 5 cu mm
Bone marrow aspirate 0.5 mL
Sample type Body fluid, unfixed tissue from normally sterile sites, bone marrow aspirate
Preferred volume
Peritoneal fluid 20 mL
Non-peritoneal fluid 25 mL
Tissue 5 cu mm
Bone marrow aspirate 0.5 mL
Min. Volume
Peritoneal fluid 10 mL
Non-peritoneal fluid 2 mL
Tissue 3 cu mm
Bone marrow aspirate 0.5 mL
UCSF Rejection Criteria Swabs not received in transport medium.
Processing notes Maintain specimen at room temperature.
Normal range No growth
Critical value Positive Gram stain
Synonyms Surgical tissue culture, bone marrow culture
Stability Room temperature 12 hours
Turn around times Up to 10 days
Reflex? If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed. Full ID and/or susceptibility testing may be omitted upon request when sample is submitted for testing.
Additional information Used for normally sterile fluids except blood, urine or CSF (e.g. joint, pericardial, peritoneal, pleural or vitreous). Includes aerobic and anaerobic cultures and Gram stain.
CPT coding 87205, 87070, 87075
Last Updated 11/12/2013 9:55:45 AM
Entry Number 1565
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