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Lab Manual for Moffitt-Long and Mount Zion

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Vaginal smear for Bacterial vaginosis/yeast (New test)

Item Value
Approval req'd? No
Available Stat? No
Test code P058
Performed by Mirobiology
In House Availability Daily, day and evening shifts only
Method Gram stain and microscopy
Collection Instructions Prepare thin smears of vaginal discharge on 2 slides, or collect swab in Amies charcoal transport medium with charcoal.
Container type Glass slides or swab in Amies Transport Media with charcoal
Amount to Collect 2 smeared slides or single swab
Sample type Vaginal discharge
Preferred volume 2 smeared slides or single swab
Units Nugent score
Normal range Nugent score < 7 without Clue cells or Nugent score < 4 if Clue cells present
Synonyms BV; clue cells; G. vaginalis; Gardnerella vaginalis
Stability
Swabs Room temperature 12 hours
Slides Indefinitely
Turn around times Same or next day
Additional information Use of a scored Gram stain (Nugent score) to demonstrate whether there has been a shift in the vaginal flora from predominantly gram-positive Lactobacillus to a gram-negative flora is recommended for diagnosis of BV, rather than culture for Gardnerella vaginalis or other organism(s).

The primary reason for performing a Gram stain on vaginal secretions is to diagnose bacterial vaginosis, therefore, this test will be performed if a Gram stain is ordered.

The presence or absence of yeast, presence or absence of Clue cells, and Nugent score is reported.

Nugent score ≥ 7, or Clue cells with Nugent score ≥ 4,is consistent with the clinical diagnosis of bacterial vaginosis.
CPT coding 87205
Last Updated 9/3/2013 12:46:58 PM
Entry Number 1563

If you have additional questions regarding this test, please call: 415-353-1667

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