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Vaginal smear for Bacterial vaginosis/yeast
|In House Availability||Daily, day and evening shifts only|
|Method||Gram stain and microscopy|
|Collection Instructions||Prepare thin smears of vaginal discharge on 2 slides, or collect swab in Amies charcoal transport medium with charcoal.|
|Container type||Glass slides or swab in Amies Transport Media with charcoal|
|Amount to Collect||2 smeared slides or single swab|
|Sample type||Vaginal discharge|
|Preferred volume||2 smeared slides or single swab|
|Normal range||Nugent score < 7 without Clue cells or Nugent score < 4 if Clue cells present|
|Synonyms||BV; clue cells; G. vaginalis; Gardnerella vaginalis|
|Turn around times||Same or next day|
|Additional information||Use of a scored Gram stain (Nugent score) to demonstrate whether there has been a shift in the vaginal flora from predominantly gram-positive Lactobacillus to a gram-negative flora is recommended for diagnosis of BV, rather than culture for Gardnerella vaginalis or other organism(s).
The primary reason for performing a Gram stain on vaginal secretions is to diagnose bacterial vaginosis, therefore, this test will be performed if a Gram stain is ordered.
The presence or absence of yeast, presence or absence of Clue cells, and Nugent score is reported.
Nugent score ≥ 7, or Clue cells with Nugent score ≥ 4,is consistent with the clinical diagnosis of bacterial vaginosis.
|Last Updated||8/7/2014 9:51:02 AM|