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Busulfan Pharmacokinetics, Adult (Non-Research)

Item Value
Test Update Information Effective 04/02/2013, busulfan testing will be performed in-house at China Basin Chemistry using LC-MS/MS.

The results of this method are approximately 2% higher than the results from Mayo.

Available Stat? No
Test group Busulfan
Performed by China Basin Chemistry
In House Availability Run Monday-Friday AM with prior notice
Method Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Collection Instructions Samples should only be collected Sunday through Thursday and the collection MUST be completed and the last sample delivered to the laboratory by 9 PM in order to provide results the next day.

Testing should be ordered in APeX. Complete the busulfan information sheet in APeX including the patient's weight, dose, dose number, exact time of the start and end of the infusion and approximate draw times for each sample.

The first sample should be drawn immediately after termination of the IV infusion, label it with the patient registration label and write the EXACT time of collection on the label. Hand carry the sample to lab on ice. Make sure to notify lab staff that you are delivering a Busulfan sample, DO NOT simply leave it at the desk.

Draw additional samples at 1, 2 and 4 hours after the termination of the infusion. Label each sample with the patient registration labels and write on the label the EXACT time of collection for each sample.

Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing.

Click here Busulfan Information Sheet
Container type Dark green top on ice x4 drawn at specific times
Amount to Collect 2 mL blood for each sample
Sample type Heparinized plasma
Preferred volume 1 ml heparinized plasma for each sample
Min. Volume 1 ml heparinized plasma for each sample
UCSF Rejection Criteria Sample not received on ice. Not collected in Dark green top tube.
Processing notes Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label. Place a Sunquest label on the tube.
Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C. Contact Special Chemistry at China Basin to alert them that a Busulfan is being processed (415-353-4820).
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above.
When all four samples have been processed, send them on dry ice to China Basin Chemistry.

NOTE:The back-up laboratory for this test is the Mayo Clinic. A busulfan information sheet for the Mayo Clinic needs to be completed for each set of samples: Click here for form

Using the information entered in Apex for this patient, complete the busulfan information sheet (e.g. the dose, dose number, infusion start time, infusion stop time and EXACT collection time of each sample). Insert the pager or phone number of the doctor who ordered the busulfan testing in the results reporting box. Ship the samples on dry ice with the busulfan information sheet to the Mayo Clinic.
Busulfan concentration in each individual sample ng/mL
Steady state concentration (Css) ng/mL
Area under the curve (AUC) µmol*min
Normal range
Area Under the Curve 900-1500 µmol*min
Steady state concentration (Css) 600-900 ng/mL
Synonyms Busulfex
Before separation from cells Refrigerated (4C) for 24 hours
After separation from cells Frozen (-70C) for 2 years.
Turn around times Results available next day by 1300 hours
Additional information Chemistry China Basin will report the busulfan concentration of each sample, the Area Under the Curve (AUC) and a steady state concentration (Css).

For questions contact China Basin Chemistry at 415 353 4820
CPT coding 80299 x number of samples tested
LDT or Mod FDA? Yes
Last Updated 8/7/2014 9:50:45 AM
Entry Number 1537
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