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Busulfan Pharmacokinetics, Adult

Item Value
Test Update Information Effective 04/02/2013, busulfan testing will be performed in-house at China Basin Chemistry using LC-MS/MS.

The results of this method are approximately 2% higher than the results from Mayo.

Available Stat? No
Test code BUSUA, BUSAR2, BUSAR3, BUSAR4, BUSAR5, BUSAR6
Test group Busulfan
Performed by China Basin Chemistry
In House Availability Monday-Friday AM with prior notice (excluding holidays)
Method Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Collection Instructions Samples should only be collected Sunday through Thursday and the collection MUST be completed and the last sample delivered to the laboratory by 4AM in order to provide results the same day.

Testing should be ordered in APeX.

The first sample should be drawn immediately after termination of the IV infusion, label it with the patient registration label and write the EXACT time of collection on the label. Hand carry the sample to lab on ice. Make sure to notify lab staff that you are delivering a Busulfan sample, DO NOT simply leave it at the desk.

Draw additional samples at the times listed on the busulfan PK sheets after the termination of the infusion. Label each sample with the patient registration labels and write on the label the EXACT time of collection for each sample.

Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing.
Container type Dark green top drawn at specific times
Amount to Collect 2 mL blood for each sample
Sample type Heparinized plasma
Preferred volume 1 ml heparinized plasma for each sample
Min. Volume 1 ml heparinized plasma for each sample
UCSF Rejection Criteria Sample not received on ice. Not collected in Dark green top tube.
Processing notes Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label. Place a Sunquest label on the tube.
Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.

NOTE:The back-up laboratory for this test is the Mayo Clinic. A busulfan information sheet for the Mayo Clinic needs to be completed for each set of samples: Click here for form

Using the information entered in Apex for this patient, complete the busulfan information sheet (e.g. the dose, dose number, infusion start time, infusion stop time and EXACT collection time of each sample). Insert the pager or phone number of the doctor who ordered the busulfan testing in the results reporting box. Ship the samples on dry ice with the busulfan information sheet to the Mayo Clinic.
Units ng/mL for busulfan concentration of each individual sample
µmol*min for area under the curve (AUC)
Normal range Area under the curve therapeutic target:
Q6hr dosing: 900 – 1500 µmol*min
Q24hr dosing: 6000 µmol*min
Synonyms Busulfex
Stability
Before separation from cells Refrigerated (4C) for 24 hours
After separation from cells Frozen (-70C) for 2 years.
Turn around times Test performed Monday-Friday AM (excluding holidays) with prior notice
Additional information Chemistry China Basin will report the busulfan concentration of each sample and the area under the curve.


For questions contact China Basin Chemistry at 415 353 4820
CPT coding 80299 x number of samples drawn
LDT or Mod FDA? Yes
Last Updated 11/16/2017 5:15:32 PM
Entry Number 1537
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