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Busulfan Pharmacokinetics, Pediatric (Non-Research)

Item Value
Approval req'd? No
Available Stat? No
Test code BUSRP1, BUSRP2, BUSRP3, BUSRP4, BUSRP5, BUSRP6, BUSRP7
Test group Busulfan
Performed by China Basin Chemistry
In House Availability Run Monday-Friday AM with prior notice (excluding holidays)
Method Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Collection Instructions Samples should only be collected Sunday through Friday and the collection MUST be completed and the last sample delivered to the laboratory by 5:00AM in order to provide results the same day.

Testing should be ordered in APeX.

For PK studies on the first busulfan dose, the first sample should be drawn immediately after termination of the IV infusion. For PK studies on the steady state busulfan doses, the first sample should be drawn immediately before the start of the IV infusion. The remaining samples should be drawn at the times listed on the busulfan PK sheets (use correct PK sheet for each study: e.g. Q6hr, Q12hr or Q24hr).

Each patient sample should have a label affixed to it, and the EXACT time of collection should be written on the label or the ApeX requisition and on the busulfan PK sheet. Hand-carry the sample to lab on ice with the requisition. Make sure to notify lab staff that you are delivering a busulfan sample, DO NOT simply leave it at the desk. Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing. With the final sample, bring the busulfan PK sheet with the correct collect times completed for each sample to the lab.
Container type Dark green top on ice drawn at specific times
Amount to Collect 2 mL blood for each sample
Sample type Heparinized plasma
Preferred volume 1 ml heparinized plasma for each sample
Min. Volume 1 ml heparinized plasma for each sample
UCSF Rejection Criteria Sample not received on ice. Not collected in Dark green top tube.
Processing notes Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label or ApeX requisition. Place a Sunquest label on the tube. Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.

As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.

NOTE:The back-up laboratory for this test is at the University of Pennsylvania Toxicology Laboratory. A busulfan information sheet for the University of Pennsylvania needs to be completed for each set of samples: Click here for UPenn form


Make a copy of the busulfan PK sheet (e.g. containing the dose, dose number, infusion start time, infusion stop time and EXACT collection time of each sample). Insert the name and the pager or phone number of the doctor who ordered the busulfan testing in the results reporting box. Call the University of Pennsylvania Toxicology Laboratory at 1-215-662-3474 to let them know that we will be sending them busulfan samples. Ship the samples on dry ice with the busulfan information sheet to the University of Pennsylvania Toxicology Laboratory.
Units ng/mL
Normal range
Synonyms Busulfex
Stability
Before separation from cells Refrigerated (4C) for 24 hours
After separation from cells Frozen (-70C) for 2 years.
Turn around times Results available same day by 1300 hours
Additional information Chemistry China Basin will report the busulfan concentration of each sample.

For questions contact China Basin Chemistry at 415 353 4820
CPT coding 80299 x number of samples tested
LDT or Mod FDA? Yes
Last Updated 8/24/2016 4:44:01 PM
Entry Number 1536
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