UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for Moffitt-Long and Mount Zion

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

Juvenile Myelomonocytic Leukemia Associated Exon Panel – Family F/U

Item Value
Available Stat? No
Utilization Guidelines Tests with long turn-around times (i.e., Molecular based tests and Microarrays) should only be requested on an inpatient if the result is going to affect the inpatient management. If the patient will likely be discharged before the result will be available, the test should be requested after discharge. (NOTE: UCSF Medical Center is not reimbursed for inpatient testing).

An exception to the above may be appropriate if there is a possibility the patient will not survive to be discharged and the information is important for diagnosis and/or family decisions/management (e.g., recurrence risk).
Test code FJMML
Performed by Molecular Diagnositcs
In House Availability Run 1st and 3rd Monday of every month, day shift only
Method Next Generation Sequencing
Collection Instructions Avoid hemolysis.

Due to stability issues these samples should only be collected Monday through noon Friday
Container type
Blood or Bone marrow Lavender top (EDTA)
Germiline (non-tumor) sample T25 flask or buccal swab
Amount to Collect
Blood 5 mL
Bone marrow 3 mL
Germline (non-tumor) sample Cultured fibroblasts (confluent T25 flasks x2) or buccal swab x2
Sample type EDTA whole blood, bone marrow, cultured fibroblasts or buccal swab
Preferred volume
Blood 5 mL
Bone marrow 3 mL
Germline sample Cultured fibroblasts (confluent T25 flasks x2) or buccal swab x2
Min. Volume
Blood 3 mL
Bone marrow 2 mL
Germline sample Cultured fibroblasts (confluent T25 flasks x2) or buccal swab x2
Processing notes Do not freeze blood or bone marrow samples. Ship to CB as soon as possible.
Normal range Negative
Synonyms JMML Panel; CBL exons 8 & 9; KRAS exons 2 & 3; NRAS exons 2 & 3; PTPN11 exons 3, 4 & 13
Turn around times 2-4 weeks
Additional information An interpretation of this test by a laboratory physician will automatically be performed and billed for separately.

This sequencing test sequences one exon of the following genes: CBL exons 8 & 9; KRAS exons 2 & 3; NRAS exons 2 & 3; PTPN11 exons 3, 4 & 13

Targeted, single exon, germline and family follow-up studies will only be performed when the diagnostic mutation was determined by the UCSF Molecular Diagnostics lab, or a report from another CLIA lab can be provided. Otherwise, please order "JMML Associated Exon Panel (JMML)" for testing.

For questions, contact the Molecular Diagnostics laboratory at 415-514-8488
CPT coding 81479, except for specific mutations
Specific codons/exons Test code CPT-4
KRAS codons 12 & 13 FJMML2 81275
KRAS exon 3 FJMML3 81403
NRAS exons 1 & 2 FJMML4 81404
All others FJMML 81479
LDT or Mod FDA? Yes
Last Updated 4/15/2014 10:31:55 AM
Entry Number 1505

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center