Click here for more information about laboratory operations and procedures
RNA Polymerase III Antibody
| Item | Value |
|---|---|
| Available Stat? | No |
| Test code | RNAP |
| Performed by | Quest |
| Sendout? | Yes |
| Method | Enzyme Linked Immunosorbent Immunoassay (ELISA) |
| Container type | Red top or Gold top |
| Amount to Collect | 1.0 mL blood |
| Sample type | Serum |
| Preferred volume | 0.5 mL serum |
| Min. Volume | 0.3 mL serum |
| UCSF Rejection Criteria | Gross hemolysis |
| Ref Lab Rejection Criteria | Gross hemolysis |
| Units | Units |
| Normal range | < 20 Units |
| Stability | Room temperature 4 days, refrigerated 1 week, frozen 1 month. |
| Turn around times | 3-7 days |
| Additional information | Autoantibodies to RNA Polymerase III antigen are found in 11% to 23% of patients with systemic sclerosis. Patients who are positive for RNA Polymerase III antibodies do not have any of the other antibodies typically found in systemic sclerosis patients such as anti-centromere, anti-Scl-70, or anti-Pm/Scl antibodies. Thus, they are a separate serologic group. Numerous studies have shown that these patients have an increased risk of the diffuse cutaneous form of scleroderma, with high likelihood of skin involvement and hypertensive renal disease. Antibodies to several different types of RNA Polymerases are found in patients with systemic sclerosis. The immunodominant epitope on RNA Pol III was identified and cloned. The recombinant immunodominant epitope of RNA Pol III can be used in ELISA with high specificity to detect anti-RNA Pol III antibodies in patients with the diffuse cutaneous form of systemic sclerosis, with a high incidence of skin involvement. |
| CPT coding | 83520-90 |
| Last Updated | 5/22/2013 1:28:30 PM |
| Entry Number | 1467 |
If you have additional questions regarding this test, please call: 415-353-1667