UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu

RNA Polymerase III Antibody

Item Value
Available Stat? No
Test code RNAP
Performed by Quest
Sendout? Yes
Method Enzyme Linked Immunosorbent Immunoassay (ELISA)
Container type Red top or Gold top
Amount to Collect 1.0 mL blood
Sample type Serum
Preferred volume 0.5 mL serum
Min. Volume 0.3 mL serum
UCSF Rejection Criteria Gross hemolysis
Ref Lab Rejection Criteria Gross hemolysis
Units Units
Normal range < 20 Units
Stability Room temperature 4 days, refrigerated 1 week, frozen 1 month.
Turn around times 3-7 days
Additional information Autoantibodies to RNA Polymerase III antigen are found in 11% to 23% of patients with systemic sclerosis. Patients who are positive for RNA Polymerase III antibodies do not have any of the other antibodies typically found in systemic sclerosis patients such as anti-centromere, anti-Scl-70, or anti-Pm/Scl antibodies. Thus, they are a separate serologic group. Numerous studies have shown that these patients have an increased risk of the diffuse cutaneous form of scleroderma, with high likelihood of skin involvement and hypertensive renal disease. Antibodies to several different types of RNA Polymerases are found in patients with systemic sclerosis. The immunodominant epitope on RNA Pol III was identified and cloned. The recombinant immunodominant epitope of RNA Pol III can be used in ELISA with high specificity to detect anti-RNA Pol III antibodies in patients with the diffuse cutaneous form of systemic sclerosis, with a high incidence of skin involvement.
CPT coding 83520-90
Last Updated 5/22/2013 1:28:30 PM
Entry Number 1467
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center