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Lab Manual for Moffitt-Long and Mount Zion

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HLA Antibody Testing – Class II PRA

Item Value
Approval req'd? No
Available Stat? Yes
Test code ILPRA2
Test group HLA Antibody Testing
Performed by Immunogenetics & Transplantation Laboratory (ITL)
Sendout? Yes
Method Luminex-based
Collection Instructions Fill Red top tube completely. If being collected with other antibody and or crossmatch testing, collect 2 tubes.

Print out and submit an ITL Requisition with the sample. Click here for Form
Container type Red top
Amount to Collect 6 mL blood
Sample type Serum
Preferred volume 4 mL serum
Min. Volume 2 mL serum
Processing notes Keep sample at Room Temperature, DO NOT refrigerate or centrifuge.
Normal range
Synonyms Class I PRA by Luminex, PRA Class II
Turn around times Test run Monday – Friday. Expected TAT for routine test is < 11 working days.
Reflex? Yes
Additional information This test detects sensitization to HLA Class I antigens through blood transfusion, organ transplantation, pregnancy, etc. It is reported as a percentage of positive cells in a panel.

In addition to solid organ testing protocols, this test can be requested for evaluation of refractoriness to platelet transfusions (contact Blood Bank, 415-353-1313).
CPT coding 86831
Last Updated 2/14/2014 9:30:40 AM
Entry Number 1440

If you have additional questions regarding this test, please call: 415-353-1667

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