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Brucella antibodies (IgG, IgM)

Item Value
Approval req'd? No
Available Stat? No
Test code BRUCI
Performed by Focus via Quest
Sendout? Yes
Method Enzyme Immunoassay
Collection Instructions Avoid hemolysis.
Container type Red top (Gold top acceptable)
Amount to Collect 4 mL blood
Sample type Serum
Preferred volume 2 mL serum
Min. Volume 0.8 mL serum
Processing notes Allow sample to clot at room temperatur then centrifuge. Refrigerate aliquot. Order Quest # 10566N.
Ref Lab Rejection Criteria Hemolysis, lipemia
Units units
Normal range < 0.80 U

Interpretive criteria:
Negative < 0.80 U
Equivocal 0.80-1.09 U
Positive ≥ 1.10 U
Stability Room temperature 3 days, refrigerated 2 weeks, frozen at -20C 1 month
Turn around times Set up 2x per week. Turn around 5-7 days.
Reflex? If Brucella IgM result is ≥ 1.10, then Brucella Antibody, Agglutination will be performed at an additional charge
Additional information Acute brucellosis is characterized by the appearance of Brucella-specific IgM within the first week of infection, followed by the appearance of Brucella-specific IgG after the second week. Levels of both IgM and IgG decline slowly over several months in conjunction with recovery. Persistence of high IgG levels with declining or absent IgM suggests chronic infection or relapse. If acute infection is suspected, Brucella culture and/or antibody testing of another serum specimen (collected 1-2 weeks after this specimen) may be warranted.
CPT coding 86622-90 x2
LOINC code 44458-8
Last Updated 9/9/2013 3:17:31 PM
Entry Number 144
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