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HLA Antibody Specificity – Class II

Item Value
Approval req'd? No
Available Stat? Yes
Test code ILLS2
Test group HLA Antibody Testing
Performed by Immunogenetics & Transplantation Laboratory (ITL)
Sendout? Yes
Method Luminex-based
Collection Instructions SAMPLE COLLECTION GUIDE FOR ITL TESTS:

Please use the following guide when collecting sample for ITL:

ITL TEST CATEGORY MAX. # TUBES FOR PT's > 12 KG SAMPLE VOL. FOR PT's < 12 KG
Antibody 2 RED TOP* 2.5 mL in RED TOP
HLA Typing 2 ACD* 2.5 mL in small ACD
Chimerism 2 ACD* 2.5 mL in small ACD
Crossmatch Donor 6 ACD* CONTACT ITL
Crossmatch Recipient 1 RED TOP* CONTACT ITL
Auto Crossmatch 6 ACD* & 1 RED TOP* CONTACT ITL

* Completely full 8.5 mL tubes

If two or more test categories are ordered add the # tubes together.
ITL (415) 476-3387

Print out and submit an ITL Requisition with the sample. Click here for Form
Container type Red top
Amount to Collect 6 mL blood
Sample type Serum
Preferred volume 4 mL serum
Min. Volume 2 mL serum
UCSF Rejection Criteria Hemolyzed sample and or presence of substances that can interfere with the assay (high background).
Processing notes Keep sample at Room Temperature, DO NOT refrigerate or centrifuge.
Normal range
Synonyms Class II Single Antigen Testing by Luminex, Single Antigen Specificity Class II
Stability If kept at ambient temperature, can be good for up to 72 hours
Turn around times Test run Monday – Friday. Expected TAT for routine test is < 11 working days.
Reflex? Yes
Additional information This test assigns cPRA (calculated PRA) and determines specific Class II antibodies. It is used in both pre-transplant antibody testing and post-transplant monitoring.

This test is NOT for evaluation of transfusion-related fever or refractoriness to platelet transfusions (contact Blood Bank, 415-353-1313).
CPT coding 86833
Last Updated 2/14/2014 9:29:45 AM
Entry Number 1429
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