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Lab Manual for Moffitt-Long and Mount Zion

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HLA-A Typing – Intermediate Resolution

Item Value
Approval req'd? No
Available Stat? Yes
Test code ILALD
Test group HLA Typing
Performed by Immunogenetics & Transplantation Laboratory (ITL)
Sendout? Yes
Method Molecular – SSP/SSOP
Collection Instructions Fill ACD tube completely. Obtain ACD tube from Specimen Receiving. If being collected with other HLA intermediate resolution typing such as HLA-B, HLA-C, HLA-DR, HLA-DQ, etc., 1 tube is sufficient for all tests. Collect additional samples if white blood cell (WBC) count is low (<1,000).

SAMPLE COLLECTION GUIDE FOR ITL TESTS:

Please use the following guide when collecting sample for ITL:

ITL TEST CATEGORY MAX. # TUBES FOR PT's > 12 KG SAMPLE VOL. FOR PT's < 12 KG
Antibody 2 RED TOP* 2.5 mL in RED TOP
HLA Typing 2 ACD* 2.5 mL in small ACD
Chimerism 2 ACD* 2.5 mL in small ACD
Crossmatch Donor 6 ACD* CONTACT ITL
Crossmatch Recipient 1 RED TOP* CONTACT ITL
Auto Crossmatch 6 ACD* & 1 RED TOP* CONTACT ITL

* Completely full 8.5 mL tubes

If two or more test categories are ordered add the # tubes together.
ITL (415) 476-3387

For other specimens, contact ITL at 6-3387.

Print out and submit an ITL Requisition with the sample. Click here for Form
Container type Yellow top (ACD)
Amount to Collect 8.5 mL blood
Sample type ACD anticoagulated whole blood; contact ITL at 6-3887 for other specimens such as amniotic fluid, bone marrow aspirate, buccal mucosa, cord blood, cultured cells, DNA, whole blood spotted on filter paper, etc.
Preferred volume 8.5 mL blood; for other specimens, contact ITL at 6-3887
Min. Volume 1 mL blood; for other specimens, contact ITL at 6-3887
UCSF Rejection Criteria WBC count too low (<1,000). For other specimens, contact ITL at 6-3387.
Processing notes Keep sample at Room Temperature, DO NOT refrigerate or centrifuge. For other specimens, contact ITL at 6-3387.
Normal range
Synonyms HLA-A Typing by SSP/SSOP
Stability If kept at ambient temperature, can be good for up to 72 hours. For other specimens, contact ITL at 6-3387.
Turn around times Test run Monday – Friday. Expected TAT for routine test is < 8 working days.
CPT coding 81373
Last Updated 2/14/2014 8:33:50 AM
Entry Number 1399
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