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Lab Manual for Moffitt-Long and Mount Zion

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B-Cell Crossmatch by Cytotoxicity w/ DTT (Recipient)

Item Value
Available Stat? Yes
Test code ILBXDT
Test group HLA Crossmatching
Performed by Immunogenetics & Transplantation Laboratory (ITL)
Sendout? Yes
Method Cytotoxicity
Collection Instructions Fill Red top tube completely. If being collected with other antibody and or crossmatch testing, collect 2 tubes.

SAMPLE COLLECTION GUIDE FOR ITL TESTS:

Please use the following guide when collecting sample for ITL:

ITL TEST CATEGORY MAX. # TUBES FOR PT's > 12 KG SAMPLE VOL. FOR PT's < 12 KG
Antibody 2 RED TOP* 2.5 mL in RED TOP
HLA Typing 2 ACD* 2.5 mL in small ACD
Chimerism 2 ACD* 2.5 mL in small ACD
Crossmatch Donor 6 ACD* CONTACT ITL
Crossmatch Recipient 1 RED TOP* CONTACT ITL
Auto Crossmatch 6 ACD* & 1 RED TOP* CONTACT ITL

* Completely full 8.5 mL tubes

If two or more test categories are ordered add the # tubes together.
ITL (415) 476-3387

Print out and submit an ITL Requisition with the sample. Click here for Form
Container type Red top
Amount to Collect 6 mL blood
Sample type Serum
Preferred volume 4 mL serum
Min. Volume 2 mL serum
UCSF Rejection Criteria Hemolyzed sample
Processing notes Keep sample at Room Temperature, DO NOT refrigerate or centrifuge.
Normal range
Synonyms B-Cell Crossmatch by NIH DTT Treated
Stability If kept at ambient temperature, can be good for up to72 hours
Turn around times Test run Monday – Friday. Expected TAT for routine test is < 5 working days.
CPT coding 86805
Last Updated 2/14/2014 8:32:28 AM
Entry Number 1386

If you have additional questions regarding this test, please call: 415-353-1667

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