UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for Moffitt-Long and Mount Zion

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

HLA Antibody Screening – Mixed Beads

Item Value
Approval req'd? No
Available Stat? Yes
Test code ILANS
Test group HLA Antibody Testing
Performed by Immunogenetics & Transplantation Laboratory (ITL)
Sendout? Yes
Method Luminex-based
Collection Instructions SAMPLE COLLECTION GUIDE FOR ITL TESTS:

Please use the following guide when collecting sample for ITL:

ITL TEST CATEGORY MAX. # TUBES FOR PT's > 12 KG SAMPLE VOL. FOR PT's < 12 KG
Antibody 2 RED TOP* 2.5 mL in RED TOP
HLA Typing 2 ACD* 2.5 mL in small ACD
Chimerism 2 ACD* 2.5 mL in small ACD
Crossmatch Donor 6 ACD* CONTACT ITL
Crossmatch Recipient 1 RED TOP* CONTACT ITL
Auto Crossmatch 6 ACD* & 1 RED TOP* CONTACT ITL

* Completely full 8.5 mL tubes

If two or more test categories are ordered add the # tubes together.
ITL (415) 476-3387

Print out and submit an ITL Requisition with the sample. Click here for Form
Container type Red top
Amount to Collect 6 mL blood (See Collection Incstructions)
Sample type Serum
Preferred volume 4 mL serum
Min. Volume 2 mL serum
UCSF Rejection Criteria Hemolyzed sample and or presence of substances that can interfere with the assay (high background).
Processing notes Keep sample at Room Temperature, DO NOT refrigerate or centrifuge.
Normal range
Synonyms HLA Antibody Screening by Luminex, Class I & II Antibody Screening
Stability If kept at ambient temperature, can be good for up to 72 hours
Turn around times Test run Monday – Friday. Expected TAT for routine test is < 11 working days.
Reflex? Yes
Additional information Antibody identification (specificity) is performed at an additional charge on all positive screens (LS1 and or LS2) unless otherwise specified.

This test detects sensitization to HLA Class I and Class II antigens through blood transfusion, organ transplantation, pregnancy, etc. It is reported as Positive (POS) or Negative (NEG) for each class.

This test is NOT for evaluation of transfusion-related fever or refractoriness to platelet transfusions (contact Blood Bank, 415-353-1313).
CPT coding 86828
Last Updated 2/14/2014 8:32:03 AM
Entry Number 1383

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center