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Lab Manual for Moffitt-Long and Mount Zion

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Lyme Disease Antibody Total (EIA)

Item Value
Approval req'd? Non-serum samples, call Immunology at x3-1712 for approval
Available Stat? No
Utilization Guidelines Laboratory diagnosis of suspected Lyme disease is best performed through serology testing. PCR very rarely detects organisms, even in clinical Lyme disease, and is typically not indicated. Positive bloodstream antibodies do not necessarily indicate active Lyme disease, but do indicate previous exposure. Positive antibody testing is automatically confirmed with Western Blot.

Most patients with CNS Lyme disease have high levels of bloodstream antibodies; therefore testing of CSF for Lyme antibodies is typically only indicated for patients with positive serology. PCR for Lyme organisms in CSF has very low sensitivity for CNS disease.
Test code LYMET
Test group Borrelia burgdorferi
Performed by Immunology
In House Availability Wednesday (day shift)
Method EIA
Container type Gold top
Amount to Collect 3 mL blood
Sample type Serum
Preferred volume 1.5 mL serum
Min. Volume 0.5 mL serum
Processing notes Freeze serum at -20C
Units Index
Normal range Negative
Synonyms Borreliosis; Borrelia burgdorferi Ab; Borrelia burgdorferi Antibody
Turn around times Test run once per week Turnaround time: 2-8 days.
Reflex? Western Blot confirmation is automatically performed on all EIA positive tests.
Additional information A negative result on this test should be followed up in two to four weeks if lyme disease is still suspected. A negative result does not rule out infection with Borrelia Burgdorferi.

Due to recommendations by the FDA, all positive or equivocal Borrelia Burgdorferi antibody EIA (screening) tests will be followed by the relevant western blot.

The screening test for B. Burgdorferi has a low negative predictive value result when used to detect early infection and a low positive predictive value when exposure history, symptoms, and clinical findings are not consistent with lyme disease.

Positive or equivocal results should not be interpreted as true positives until a second-step testing of the specimen is done using method that is more specific for antibodies to B. Burgdoreri (e.g. western blot). The patient will be billed an additional charge if the Lyme Disease by Western Blot is performed.
LOINC code 20449-5
Last Updated 11/30/2012 3:17:15 PM
Entry Number 137

If you have additional questions regarding this test, please call: 415-353-1667

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