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Hepatitis B DNA, Quantitative

Item Value
Available Stat? No
Test code HBVRT
Test group Hepatitis
Performed by Immunology
Method RT-PCR
Container type Lavender top (6 mL)
Amount to Collect 6 mL blood
Sample type EDTA plasma
Preferred volume 3 mL plasma
Min. Volume 1.1 mL plasma
UCSF Rejection Criteria Gold or Red top vacutainer received. Sample not separated from cells within 6 hours of collection.
Processing notes Separate plasma within 6 hours of collection. Freeze plasma at -70C.
Units IU/mL or log IU/mL
Normal range Target not detected (< 10 IU/mL or < 1.00 log IU/mL)
Turn around times Performed 1x per week. Turnaround time 3-9 days.
Additional information The use of specimens collected in serum tubes containing Z-clot activator, or similar types of rapid clot activator, may cause inhibited results in the Real Time HBV assay.

High off-scale results are routinely reported as > 1,000,000,000 IU/mL or > 9.0 log IU/mL. .

Low level results where HBV DNA is detected by the assay but not quantifiable are reported as Detected with a result <10 IU/mL or <1.00 log IU/mL.

Target Not Detected is reported when no HBV DNA can be detected by the assay. This result should not imply the patient is not infected with HBV. Viral loads less than 10 IU/mL will not be reliably detected by this assay. Correlation with clinical findings and serologic results are recommended.

The assay is intended for use as an aid in the management of patients with chronic HBV infection undergoing anti-viral therapy. The assay can be used to measure HBV DNA levels at baseline and during treatment to aid in assessing response to treatment. This assay is not intended for use as a screening test in blood or blood products for HBV or as a diagnostic test to confirm the presence of HBV infection.
CPT coding 87517
Last Updated 2/19/2015 2:14:24 PM
Entry Number 1366
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