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B. pertussis/parapertussis PCR
| Item | Value |
|---|---|
| Approval req'd? | No |
| Available Stat? | No |
| Test code | P355 |
| Performed by | Focus Diagnostics |
| Sendout? | Yes |
| Method | Real time PCR |
| Collection Instructions | Collect nasal aspirate or washing as described in the Microbiology section under the 'General lab Information' link but clear the tubing of aspirated material by suctioning or transfer of the nasal wash into a sterile container. Deliver the sample to the laboratory immediately post-collection. |
| Container type | Sterile container |
| Amount to Collect | 1 mL nasal wash or aspirate |
| Sample type | Nasal wash or aspirate |
| Preferred volume | 1 mL nasal wash or aspirate |
| Min. Volume | 0.5 mL nasal wash or aspirate |
| Normal range | Not Detected |
| Critical value | Positive for Bordetella spp. |
| Synonyms | Whooping Cough; Haemophilus pertussis; Bordetella pertussis PCR; Bordetella parapertussis; Bordetella pertussis DNA |
| Stability | Frozen at -70C, 30 days |
| Turn around times | 3-5 days |
| Additional information | This assay is based upon the use of real-time PCR amplification of specific genomic DNA sequences to differentially detect B. pertussis and B. parapertussis. This is the most sensitive and specific assay for the presence of B. pertussis, and is preferable to direct FA and culture, which are no longer offered; each of the latter is about 50% as sensitive and in combination are only 60-70% as sensitive. |
| CPT coding | 87798 x2 |
| LOINC code | 43913-3 |
| Last Updated | 3/24/2013 7:07:58 PM |
| Entry Number | 135 |
If you have additional questions regarding this test, please call: 415-353-1667