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Lab Manual for Moffitt-Long and Mount Zion

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B. pertussis/parapertussis PCR

Item Value
Approval req'd? No
Available Stat? No
Test code P355
Performed by Focus Diagnostics
Sendout? Yes
Method Real time PCR
Collection Instructions Collect nasal aspirate or washing as described in the Microbiology section under the 'General lab Information' link but clear the tubing of aspirated material by suctioning or transfer of the nasal wash into a sterile container. Deliver the sample to the laboratory immediately post-collection.
Container type Sterile container
Amount to Collect 1 mL nasal wash or aspirate
Sample type Nasal wash or aspirate
Preferred volume 1 mL nasal wash or aspirate
Min. Volume 0.5 mL nasal wash or aspirate
Normal range Not Detected
Critical value Positive for Bordetella spp.
Synonyms Whooping Cough; Haemophilus pertussis; Bordetella pertussis PCR; Bordetella parapertussis; Bordetella pertussis DNA
Stability Frozen at -70C, 30 days
Turn around times 3-5 days
Additional information This assay is based upon the use of real-time PCR amplification of specific genomic DNA sequences to differentially detect B. pertussis and B. parapertussis.

This is the most sensitive and specific assay for the presence of B. pertussis, and is preferable to direct FA and culture, which are no longer offered; each of the latter is about 50% as sensitive and in combination are only 60-70% as sensitive.
CPT coding 87798 x2
LOINC code 43913-3
Last Updated 3/24/2013 7:07:58 PM
Entry Number 135

If you have additional questions regarding this test, please call: 415-353-1667

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