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Lab Manual for Moffitt-Long and Mount Zion

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Hepatitis C RNA, Quantitative by Real Time PCR

Item Value
Approval req'd? No
Available Stat? No
Test code HCVRT
Test group Hepatitis
Performed by Immunology
Method RT-PCR
Collection Instructions Transport to laboratory as soon as possible after collection. Sample needs to be processed within 6 hours.
Container type Lavender top 6 mL
Amount to Collect 6 mL blood
Sample type EDTA Plasma
Preferred volume 3 mL plasma
Min. Volume 1.5 mL plasma
Processing notes Separate plasma from cells within 6 hours of collection and freeze at -20C. Transport on dry ice to China Basin
Units IU/mL and log IU/mL
Normal range Target not detected (< 12 IU/mL or < 1.08 log IU/mL)
Synonyms Hepatitis C RNA, Qualitative, PCR; Hepatitis C RNA, Quantitative, PCR; HCV; Hepatitis C RNA, Quantitative by B-DNA;
Stability Room temperature 6 hours
Turn around times Performed once per week. Turnaround time 3-9 days
Additional information High off-scale results are routinely reported as > 100,000,000 IU/mL or > 8.0 log IU/mL.

Low level results where HCV RNA is detected by the assay but not quantifiable are reported as Detected with a result <12 IU/mL or <1.08 log IU/mL.

Target Not Detected is reported when no HCV RNA can be detected by the assay. This result should not imply the patient is not infected with HCV. Viral loads less than 12 IU/mL will not be reliably detected by this assay. Correlation with clinical findings and serologic results are recommended.

This test may be used as a marker for the effect of interferon or other therapy, but should not be used for diagnosis without confirmation by other established means. Positive results are automatically forwarded to California public health per regulations. However, this does not absolve the ordering provider from any additional reporting if such is required.
CPT coding 87522
Last Updated 8/15/2012 1:17:57 PM
Entry Number 1342

If you have additional questions regarding this test, please call: 415-353-1667

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