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Lab Manual for Moffitt-Long and Mount Zion

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XX/XY FISH

Item Value
Approval req'd? No
Available Stat? No
Test code XXXY
Test group FISH
Performed by Medical Genomics - Cytogenetics
Method Fluorescent in-situ hybridization
Collection Instructions Click here for Neoplastic Cytogenetics Requisition
Container type Blood & bone marrow aspirate: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844. Dark Green top also acceptable

Bone marrow core: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect
Bone marrow 2 mL
Blood 3 mL
Bone core 2 cm
Amniotic fluid 10 mL
CVS 10 mg
POC 10 mg
Sample type Heparinized whole blood, bone marrow, bone core, Amniotic fluid, CVS, POC
Preferred volume
Bone marrow 2 mL
Blood 3 mL
Bone core 2 cm
Amniotic fluid 10 mL
CVS 10 mg
POC 10 mg
Min. Volume
Bone marrow 1 mL
Blood 1mL
Bone core 1 cm
Amniotic fluid 5 mL
CVS 5 mg
POC 5 mg
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes Maintain sample at room temperature. Transport to CB Cytogenetics within 24 hours
Normal range
Synonyms Sex mismatch; Transplant monitoring; TX monitoring
CPT coding 88275, 88271
LDT or Mod FDA? Yes
Last Updated 1/22/2014 8:57:22 AM
Entry Number 1331
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