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PTEN Deletion by FISH

Item Value
Available Stat? No
Test code
Test group Oncology FISH
Performed by Medical Genomics - Molecular Diagnostics
In House Availability Run 2x per week, Monday & Wednesday, day shift only
Method Fluorescent in-situ hybridization (FISH)
Collection Instructions For UCSF Samples (from remote sites) Click here for sample collection instructions

For NON-UCSF Samples Click here for Requisition form & Account set-up instructions. Note we only do institutional billing.
Container type
Sample type Formalin-fixed, paraffin-embedded tissue on three (3) unstained slides (5 microns thick) on charged glass. One adjacent hematoxylin and eosin stained (H&E) slide should also be included. Slides should be labeled with pathology case number and block identification.
UCSF Rejection Criteria All required slides not included. Insufficient tumor present on slide as determined by pathologist. Slides not labeled or not accompanied by completed requisition form.
Units Ratio and number of PTEN and CEP10 signals
Normal range Normal Range: Ratio >0.8 (no deletion of PTEN); Signals >1.6 (no loss of chromosome 10)
Stability Slides are stable indefinitely at room temperature
Turn around times 10-14 days
Additional information An interpretation of this test by a laboratory physician will automatically be performed and billed for separately.

PTEN deletion by FISH is designed to detect either a deletion of the PTEN locus or loss of the entire 10th chromosome. This feature is seen in glioblastoma, especially the small cell variant.

The clinical interpretation of this test should be evaluated within the context of the patient's medical history, other diagnostic tests, and the histologic and immunohistochemical features of the tumor.

The test was validated by UCSF Clinical Laboratories to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
CPT coding 88368 x2
LDT or Mod FDA? Yes
Last Updated 1/22/2014 9:00:05 AM
Entry Number 1321
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