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Lymphocyte T-cell subsets, Naive and Memory

Item Value
Available Stat? No
Test code RARO
Test group CD
Performed by Immunology
In House Availability Monday-Friday (day shift). Samples must arrive in lab by noon on Friday.
Method Flow cytometry
Collection Instructions Collect only Monday - Friday. Samples must arrive in lab no later than 1200 hours on Friday or they cannot be processed.
Container type Lavender top
Amount to Collect 3 ml blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
Min. Volume 1 mL blood
UCSF Rejection Criteria Refrigerated sample. Sample > 48 hours old.
Processing notes DO NOT refrigerate, store at room temperature and ship to China Basin.

Order CBCD and THI if not already ordered on the same sample.

Samples received on weekends, holidays or after 1200 hours on Friday cannot be processed or saved.
Units % and x106cells/L
Normal range ≥ 18 year olds:
Subset Percentage Absolute
CD4+ CD45 RA+ 3-59% of CD4 T cells of CD4 T-cells 48-632 x106cells/L
CD4+ CD45 RO+ 31-76% of CD4 T cells 220-833 x106cells/L
CD8+ CD45 RA+ 15-75% of CD8 T cells 27-457 x106cells/L
CD8+ CD45 RO+ 11-65% of CD8 T cells 48-400 x106cells/L


For pediatric ranges please see following ranges established by Mayo Clinic - Department of Laboratory Medicine and Pathology; Rochester, MN 55905 (Laboratory Test Abstract Form - F 006284)

Pediatric:
CD4+ CD45 RA+ 21-75% of CD4 T cells 138-893 x106cells/L
CD4+ CD45 RO+ 11-44% of CD4 T cells 56-411 x106cells/L
CD8+ CD45 RA+ 23-88% of CD8 T cells 83-653 x106cells/L
CD8+ CD45 RO+ 2-26% of CD8 T cells 10-142 x106cells/L


Synonyms CD45RO; CD45RA; CD45RA/RO; CD45RA/CD45RO; flow cytometry
Stability Room temperature 48 hrs
Turn around times 2-3 days
Additional information Absolute cell counts require a CBC w/Differential and THI/TBHS on the same sample, which will be ordered and charged separately if not otherwise available.
LOINC code 20631-8
LDT or Mod FDA? Yes
Last Updated 11/27/2012 5:24:31 PM
Entry Number 1274
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