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Lab Manual for Moffitt-Long and Mount Zion

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Protein, Total, random urine

Item Value
Approval req'd? No
Available Stat? No
Test code TPCUR
Test group Total protein
Performed by Parnassus & Mt. Zion Chemistry
In House Availability Test available 24 hours per day 7 days per week
Method Spectrophotometric (pyrogallol red)
Container type Urine cup
Amount to Collect 20 mL urine
Sample type Random urine
Preferred volume 2 mL urine
Min. Volume 1 mL urine
Processing notes Order both TPCUR and CRUR on the sample.
Units mg/dL, mg/mg creatinine
Normal range < 0.16 mg/mg creatinine
Synonyms TP; Albumin
Stability Refrigerated 2 days
Turn around times 4-14 hours
Additional information A creatinine is performed on the same sample to calculate the protein/creatinine ratio and will be reported and billed.

In adults, a urine protein/Cr ratio > 0.16 suggests increased proteinuria and a ratio > 3.0 suggests nephrotic range proteinuria (these suggested cutoffs are based on Lane C et al., Nephrology 2006; 11, 245–249, Price C, et all, Clinical Chemistry 2005; 51:1577–1586, and consultation with UCSF Nephrology). In children, a ratio of ≥0.1 has been proposed for proteinuria and > 1.0 for nephrotic range proteinuria (Abitbol C, et al., J Pediatrics 1990; 116:243-247), however, other references indicate that results can vary greatly according to sex and body size and that a single cutoff may not be appropriate for pediatric age groups (Mori Y et al., Pediatr Nephrol 2006; 21: 683–687).

Total urine protein is determined by the pyrogallol red method using a human albumin standard. This method is effective in detecting albumin and non- albumin proteins, including immunoglobulins. 24 hour urine protein excretion may be increased in a variety of conditions including renal disease, Bence Jones proteinuria, amyloidosis, hemorrhage, systemic infections, heart failure, dehydration, starvation, strenuous exercise, pregnancy, sickle cell disease, exposure to extreme cold and psychological stress.

Comparison of measured urine protein reading to dipstick 'scale' reading:

Scale Approx. Concentration
Neg Neg
Trace < 30 mg/dL
1+ 30 mg/dL
2+ 100 mg/dL
3+ 300 mg/dL
4+ ≥ 2000 mg/dL
LOINC code 2888-6
Last Updated 3/24/2013 7:33:48 PM
Entry Number 1269
Lab Procedure Link Click here for Procedure

If you have additional questions regarding this test, please call: 415-353-1667

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