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Lab Manual for Moffitt-Long and Mount Zion

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Bilirubin, Total, Body Fluid

Item Value
Approval req'd? No
Available Stat? Yes
Test code BILTBF
Test group Bilirubin
Performed by Parnassus Chemistry
In House Availability Continuous daily.
Method Spectrophotometric (diazo)
Collection Instructions Wrap collection tube in foil to protect from light
Container type Red top or clean container
Amount to Collect 5 ml fluid
Sample type Body fluid
Preferred volume 1 mL fluid
Min. Volume 0.2 mL fluid
Units mg/dL
Normal range
Turn around times Stat: 1 hour, Routine: 4 hours
Additional information To convert mg/dL to µmol/L (SI units) multiply by 17.1. Hemolysis may artifactually increase the result; lipemia may decrease the result.

Body fluid bilirubin levels are sometimes used to investigate the possibility of bile leaks or bile peritonitis. Although there are no reference ranges available, one recent study suggests that a ratio of the bilirubin concentration in Jackson Pratt drain fluid to the bilirubin concentration in serum of greater than 5.0 is indicative of a bile leak.

Darwin, PE, Goldberg, EM, and Uradomo, LT. (2008). Jackson Pratt Drain Fluid to Serum Bilirubin Concentration Ratio for the Diagnosis of Bile Leaks. Gastrointestinal Endoscopy 67(5): AB159.

Ascitic fluid bilirubin levels have also been examined in patients with various forms of ascites. An ascitic fluid bilirubin concentration greater than 6 mg/dL and an ascitic fluid to serum bilirubin ratio of greater than 1.0 appears to be consistent with bile peritonitis.

Runyon, BA. (1987). Ascitic fluid bilirubin concentration as a key to choleperitoneum. J Clin Gastroenterol 9(5): 543-545.
Last Updated 3/24/2013 8:15:31 PM
Entry Number 1212
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