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Euglobulin Clot Lysis Time
|Patient Preparation||Patients should avoid exercise prior to collecting samples.|
|Collection Instructions||1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.
2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.
3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.
4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.
Do not massage vein vigorously, pump fist excessively or leave tourniquet in place for a prolonged period.
|Container type||Blue tops x2|
|Amount to Collect||2 blue tops filled to full extent of vacuum|
|Sample type||Citrated plasma|
|Preferred volume||2 mL|
|Min. Volume||1 mL|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected|
|Processing notes||Deliver specimen immediately to Hematology M524. Centrifuge within 30 minutes after collection to get platelet poor plasma and freeze aliquot at -20C. Order Quest test #462N|
|Normal range||> 60 minutes|
|Stability||Refrigerated 4 hours, frozen at -20C 3 weeks.|
|Turn around times||Set up once per week. Turnaround time 3-10 days|
|Additional information||The euglobulin fraction of plasma, prepared by dilution and acidification, contains fibrinogen, plasminogen, active plasmin, and plasminogen activators. The process of dilution and acidification partially depletes inhibitors of fibrinolysis, including plasminogen activator inhibitor-1 (PAI-1). The time taken for the euglobulin clot to lyse is referred to as the euglobulin clot lysis time. The euglobulin clot lysis time provides some assessment of the fibrinolytic system because the time may be decreased in a subset of patients with low levels of inhibitors of fibrinolysis. Although not well documented, it has been suggested that the euglobulin clot lysis time may be useful in confirming a deficiency of PAI-1 function when a low PAI-1 activity has been observed in the presence of normal PAI-1 antigen.|
|Last Updated||7/7/2011 8:56:30 PM|