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Lab Manual for Moffitt-Long and Mount Zion

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Respiratory Virus Panel PCR

Item Value
Approval req'd? No
Available Stat? No
Utilization Guidelines Please review the testing algorithm on test this entry.
Test code P350
Performed by Microbiology
In House Availability Test performed 3x per week
Method Multiplex Reverse Transcription PCR
Collection Instructions Nasopharyngeal swab: Use flocked swab/Universal Transport Medium for collection. Insert swab into the nostril, gently rotating the swab inward until resistance is met at the level of the turbinates. Rotate the swab a few times against the nasopharyngeal wall (approximately 10 sec) and then withdraw swab. Insert swab into container with Universal Transport Medium. Break end of swab so top of vial can be screwed on securely. Appropriately label specimen and send to the laboratory.
Container type
Nasopharyngeal swab Flocked swab in Universal Transport Medium (UTM)
Other samples Clean container
Amount to Collect
Nasopharyngeal swab 1 flocked swab
Fluid 2 mL
Sample type Nasopharyngeal swab (preferred), nasal wash or aspirate, trachael aspirate, BAL, bronchial wash
Preferred volume
Nasopharyngeal swab 1 flocked swab
Fluid 2 mL
Min. Volume
Nasopharyngeal swab 1 flocked swab
Fluid 1 mL
UCSF Rejection Criteria Nasopharyngeal swab not collected using flocked swab/UTM kit.
Normal range Not detected
Critical value Positive for Influenza or positive for RSV on inpatients and Emergency Department. Any H1N1 positives
Synonyms RSV A; RSV B; Influenza A; Influenza A subtype H1; Influenza A subtype H3; Influenza B; Parainfluenza 1; Parainfluenza 2; Parainfluenza 3; Human Metapneumovirus; Rhinovirus; Adenovirus.
Stability Refrigerated 1 week, frozen at -70C 1 month
Turn around times 3-5 days
Additional information Detects RSV A, RSV B, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza B, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, Human Metapneumovirus, Rhinovirus, and Adenovirus.

Negative results do not preclude respiratory virus infection and should not be used as the sole basis for diagnosis, treatment or other management decisions.

Testing of specimens other than NP swabs falls outside of the approved manufacturer's specimen recommendation as prescribed in the xTAG(R)-RVP package insert. The performance of this assay has been determined by the UCSF laboratory as acceptable for alternative respiratory specimen types such as BAL, bronchial wash, endotrachael aspirate, etc. Results should be used in conjunction with clinical findings.

This assay cannot adequately detect Adenovirus species C, or serotypes 7a and 41.

The primers for detection of Rhinovirus have been shown to cross-react with Enterovirus.
CPT coding 87633
LOINC code 50219-5
LDT or Mod FDA? Yes
Last Updated 7/31/2013 9:19:22 AM
Entry Number 1195
Image caption UCSF respiratory virus testing algorithm
Image Image of the UCSF respiratory virus testing algorithm
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