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Respiratory Virus Panel by PCR

Item	Value
Approval req'd?	No
Available Stat?	No
Utilization Guidelines	Please review the testing algorithm on test this entry.
Test code	P350
Performed by	Microbiology
In House Availability	Test performed 3x per week
Method	Multiplex Reverse Transcription PCR
Collection Instructions	Nasopharyngeal swab: Use flocked swab/Universal Transport Medium for collection. Insert swab into the nostril, gently rotating the swab inward until resistance is met at the level of the turbinates. Rotate the swab a few times against the nasopharyngeal wall (approximately 10 sec) and then withdraw swab. Insert swab into container with Universal Transport Medium. Break end of swab so top of vial can be screwed on securely. Appropriately label specimen and send to the laboratory.
Container type	Nasopharyngeal swab Flocked swab in Universal Transport Medium (UTM) Other samples Clean container
Amount to Collect	Nasopharyngeal swab 1 flocked swab Fluid 2 mL
Sample type	Nasopharyngeal swab (preferred), nasal wash or aspirate, trachael aspirate, BAL, bronchial wash
Preferred volume	Nasopharyngeal swab 1 flocked swab Fluid 2 mL
Min. Volume	Nasopharyngeal swab 1 flocked swab Fluid 1 mL
UCSF Rejection Criteria	Nasopharyngeal swab not collected using flocked swab/UTM kit.
Normal range	Not detected
Critical value	Positive for Influenza or positive for RSV on inpatients and Emergency Department
Synonyms	RSV A; RSV B; Influenza A; Influenza A subtype H1; Influenza A subtype H3; Influenza B; Parainfluenza 1; Parainfluenza 2; Parainfluenza 3; Human Metapneumovirus; Rhinovirus; Adenovirus.
Stability	Refrigerated 1 week, frozen at -70C 1 month
Turn around times	3-5 days
Additional information	Detects RSV A, RSV B, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza B, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, Human Metapneumovirus, Rhinovirus, and Adenovirus. Negative results do not preclude respiratory virus infection and should not be used as the sole basis for diagnosis, treatment or other management decisions. Testing of specimens other than NP swabs falls outside of the approved manufacturer's specimen recommendation as prescribed in the xTAG(R)-RVP package insert. The performance of this assay has been determined by the UCSF laboratory as acceptable for alternative respiratory specimen types such as BAL, bronchial wash, endotrachael aspirate, etc. Results should be used in conjunction with clinical findings. This assay cannot adequately detect Adenovirus species C, or serotypes 7a and 41. The primers for detection of Rhinovirus have been shown to cross-react with Enterovirus.
CPT coding	87633
LOINC code	50219-5
Last Updated	4/17/2013 2:14:10 PM
Entry Number	1195
Image caption	UCSF respiratory virus testing algorithm
Image

If you have additional questions regarding this test, please call: 415-353-1667