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Temporarily unavailable as of 11/21/2011
Plasminogen Activator Inhibitor -1 Activity

Item Value
Approval req'd? No
Available Stat? No
Utilization Guidelines If elevated PAI-1 levels are sought, PAI-1 Antigen is recommended, NOT PAI-1 activity.

In instances where the PAI-1 activity is below 2.0 IU/mL and the patient has a hemorrhagic disorder, a euglobulin clot lysis assay is recommended. An euglobulin clot lysis shorter than 60 minutes supports PAI-1 deficiency.
Test code PAI1
Test group Plasminogen
Performed by Quest
Sendout? Yes
Method Chromogenic
Patient Preparation Morning sample after fasting is required.
Collection Instructions 1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.

2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.

3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.

4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.

The venipuncture must be clean, with no trauma. The first 5 mL of blood drawn from a patient should not be used for coagulation testing. If drawn through an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected before collecting the specimen for coagulation testing.

Blood should not be collected from heparinized lines.
Container type Blue top filled to full extent of vacuum
Amount to Collect 2.7 mL blood
Sample type Citrated plasma
Preferred volume 1 mL plasma
Min. Volume 0.5 mL plasma
UCSF Rejection Criteria Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected
Processing notes Aliquot separated plasma into 1 mL sample and freeze in plastic tubes at -20C. Platelet contamination of a test sample will tend to falsely elevate results. Ship on dry ice to China Basin sendout M-F for processing to Quest or LabCorp. Order Quest test #10491, if B/ T patient order BTMOLT, order LabCorp test #146787.
Ref Lab Rejection Criteria Thawed sample received.
Units IU/mL
Normal range
≥ 18 year old males 2.0-47.1 IU/mL
≥ 18 year old females 2.0-40.7 IU/mL
Synonyms PAI-1 activity
Stability Room temperature 2 hours, refrigerated 8 hours, frozen at -20C 3 months.
Turn around times Test set up 2x per week. Turnaround 6-8 days
Additional information Increased activity may be associated with increased risk of arterial thrombosis (such as with unexplained premature myocardial infarction) and venous thrombosis.

As an acute phase reactant, the activity is increased after an acute event. Elevated levels may also be seen in diabetes and pregnancy.
CPT coding 95415-90
LOINC code 5975-8
Last Updated 11/17/2011 4:17:59 PM
Entry Number 1167
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