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PML/RARA (15;17) translocation FISH
|Performed by||Medical Genomics - Cytogenetics|
|Method||Fluorescent in-situ hybridization|
|Collection Instructions||Bone marrow is the preferred specimen, but heparinized peripheral blood may be submitted if a large number of malignant cells are present.
Collect bone marrow in a syringe, transfer to Dark Green top vacutainer and gently invert the tube several times for good mixing.
If a dry tap is obtained, consult the Laboratory Medicine resident in Hematology regarding the possible submission of a green top tube of
Contact Hematology if the specimen is more than 24 hours old.
Complete and submit a "Cytogenetics" form and send with the samples.
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|Container type||Blood & bone marrow aspirate: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844. Dark Green top also acceptable
Bone marrow core: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
|Amount to Collect||
|Sample type||Heparinized whole blood, bone marrow, bone core|
|UCSF Rejection Criteria||Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.|
|Processing notes||Maintain sample at room temperature. Transport to CB Cytogenetics within 24 hours|
|Normal range||Not detected|
|Synonyms||Cytogenetic analysis; Karyotype; Karyotyping|
|Turn around times||7-14 days|
|CPT coding||88275, 88271x2|
|LDT or Mod FDA?||Yes|
|Last Updated||1/22/2014 8:57:18 AM|
If you have additional questions regarding this test, please call: 415-353-1667