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Trisomy 8 FISH

Item Value
Approval req'd? No
Available Stat? No
Test code TRIS8
Performed by Medical Genomics - Cytogenetics
Method Fluorescent in-situ hybridization
Collection Instructions Bone marrow is the preferred specimen, but heparinized peripheral blood may be submitted if a large number of malignant cells are present.

Collect bone marrow in a syringe, transfer to Dark Green top vacutainer and gently invert the tube several times for good mixing.

If a dry tap is obtained, consult the Laboratory Medicine resident in Hematology regarding the possible submission of a green top tube of
peripheral blood.

Contact Hematology if the specimen is more than 24 hours old.

Complete and submit a "Cytogenetics" form and send with the samples.
Click here for Neoplastic Cytogenetics Requisition
Container type Blood & bone marrow aspirate: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844. Dark Green top also acceptable

Bone marrow core: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect
Bone marrow 2 mL
Blood 2 mL
Bone core 2 cm
Sample type Heparinized whole blood, bone marrow, bone core
Preferred volume
Bone marrow 2 mL
Blood 2 mL
Bone core 2 cm
Min. Volume
Bone marrow 1 mL
Blood 1 mL
Bone core 2 cm
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes Maintain sample at room temperature. Transport to CB Cytogenetics within 24 hours
Normal range Not detected
Synonyms +8; Cytogenetic analysis; Karyotype; Karyotyping
Stability 48 hours
Turn around times 7-14 days
CPT coding 88275, 88271
LOINC code 21773-7
LDT or Mod FDA? Yes
Last Updated 1/22/2014 8:57:18 AM
Entry Number 1131
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