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Lab Manual for Moffitt-Long and Mount Zion

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POCT pH, fluid

Item Value
Approval req'd? No
Available Stat? Yes
Test code
Test group pH
Performed by Authorized Point of Care testing site staff
In House Availability Test available 24 hours per day 7 days per week
Method pH paper (Hydrion 1-12)
Collection Instructions Use approx. a 2 inch strip of paper for each test.

Vaginal fluid:
Using a vaginal speculum, part the labia exposing the cervix and carefully insert the paper into the vagina. Do not allow the pH paper to come into contact with vaginal tissue during entry. Allow first and only contact to the test paper to occur with upper vaginal tissue (posterior vaginal fornix and external cervical os).

Ocular fluid:
With care, apply the tip of the paper to pooled ocular fluid. Avoid direct contact with eye
tissue.

Gastric fluid:
Apply the tip of the paper to aspirated gastric fluid. Avoid contact with fluids other than
that being tested.
Container type N/A
Amount to Collect N/A, direct application of patient sample to paper
Sample type Vaginal fluid, Ocular fluid, Gastric fluid
Preferred volume N/A, direct application of patient sample to paper
Normal range Results are reported in pH units as indicated from the color reaction of the paper. The colors below are descriptive only. The paper must be read against the pH color chart in the dispenser

Color Approx. pH
Yellow 4.5
Yellow-Green 5.0
Citrus Green 5.5
Apple Green 6.0
Green 6.5
Forest Green 7.0
Zucchinni Green 7.5
Midnight Blue Teal 8.0
Dark Midnight Blue 8.5



For vaginal fluid a pH > 6 indicates the presence of amniotic fluid and possible rupture of membranes.
Synonyms ocular pH; vaginal pH; Amniotic fluid pH; gastric pH; SROM; Spontaneous rupture of membranes
Stability Samples should be tested immediately
Additional information pH paper, used in the detection of vaginal, ocular or gastric pH, is intended for use by qualified medical and nursing staff and is intended as an aid to professional treatment.

pH paper can only indicate a pH value and should be used only as a monitoring tool.

Antibiotic therapy or infections of the vagina can lead to elevated vaginal pH resulting in a false interpretation of the presence of amniotic fluid. Where doubt exists, standard microbiological testing should be employed to exclude infection. Additionally, pH testing cannot distinguish amniotic fluid from urine. In instances where there is the possibility of urine contamination and/or where the patient has received antibiotic therapy, "fern" testing may be of value to verify the presence of amniotic fluid.
LDT or Mod FDA? No
Last Updated 11/21/2013 3:35:42 PM
Entry Number 1125

If you have additional questions regarding this test, please call: 415-353-1667

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