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Lab Manual for Moffitt-Long and Mount Zion

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POCT Hemoglobin

Item Value
Approval req'd? No
Available Stat? Yes
Test code
Test group Hemoglobin
Performed by Authorized Point of Care testing site staff
In House Availability Test available 24 hours per day 7 days per week
Method Optical detection of azidemethemoglobin production (Hemocue), calculated from impedence Hct (iStat)
Collection Instructions Fingerstick sample:

1. Wipe away the first drop of blood.
2. Apply light pressure to the finger until a drop of blood forms. Do not squeeze the finger.

3. Hold the microcuvette by the wings with the pointed end away from you.
4. Touch the pointed tip of the microcuvette to the drop of blood.
5. Allow the microcuvette to fill by capillary action
Container type Hemocue cuvette
Amount to Collect
Fingerstick 0.01 mL blood (10 µL)
Vacutainer sample 1 mL blood
Sample type Fingerstick, EDTA or Heparinized whole blood
Preferred volume
Fingerstick 0.01 mL blood (10 µL)
Vacutainer sample 1 mL blood
Min. Volume 0.01 mL blood (10 µL) for fingerstick, 1 mL blood for vacutainer samples
Units g/dL
Normal range
0-7 days 14.5-22.5 g/dL
8-14 days 13.5-21.5 g/dL
2-4 weeks 12.5-20.5 g/dL
1-2 months 10.0-18.0 g/dL
2-3 months 9.0-14.0 g/dL
3-6 os 9.5-13.5 g/dL
6-24 months 11.0-13.5 g/dL
2-5 years 11.2-13.5 g/dL
5-8 years 11.4-15.5 g/dL
8-12 years 11.6-15.5 g/dL
Male 12-15 years 12.3-16.0 g/dL
Male 15-18 years 12.6 -17.0 g/dL
Male > 18 years 13.6-17.5 g/dL
Female 12-15 years 11.8-15.5 g/dL
Female > 15 years 12.0-15.5 g/dL
Critical value ≤ 7 g/dL
Synonyms Hemocue; Hgb; Hb
Additional information Air bubbles in the microcuvette will result in erroneously low values. The microcuvette should be inspected for bubbles before testing.

The microcuvette should be filled in a continuous process. It should never be topped off after the initial filling.

Blood inside the HemoCue Classic will interfere with hemoglobin measurement.

Blood collected in vacutainer tubes with liquid anticoagulant may give erroneous readings due to the effects of dilution.
LDT or Mod FDA? No
Last Updated 5/22/2013 1:56:19 PM
Entry Number 1110
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